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Literature summary for 3.1.1.73 extracted from

  • Abokitse, K.; Wu, M.; Bergeron, H.; Grosse, S.; Lau, P.C.
    Thermostable feruloyl esterase for the bioproduction of ferulic acid from triticale bran (2010), Appl. Microbiol. Biotechnol., 87, 195-203.
    View publication on PubMed
Search for more literature for 3.1.1.73

Application

Application Comment Organism
industry bioproduction of ferulic acid from triticale bran by alkaline and thermostable FAE Caldanaerobacter subterraneus subsp. tengcongensis

Cloned(Commentary)

Cloned (Comment) Organism
777-bp DNA fragment digested with EcoRI and PstI and ligated into the corresponding sites of the IPTG-inducible plasmid pKK223-3, transformed into Escherichia coli strain JM109 and expressed from pKTtFAE vector Caldanaerobacter subterraneus subsp. tengcongensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 methyl p-coumarate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.29
-
 methyl sinapate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.32
-
 methyl ferulate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29100
-
 sequence analysis Caldanaerobacter subterraneus subsp. tengcongensis
33000
-
 1 * 33000, SDS-PAGE, purified enzyme Caldanaerobacter subterraneus subsp. tengcongensis
36000
-
 native enzyme Caldanaerobacter subterraneus subsp. tengcongensis

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis
-
-
-
Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to apparent homogeneity by centrifugation, ammonium sulfate precipitation, ultrafiltration and gel filtration, 30.5fold with a yield of 9% Caldanaerobacter subterraneus subsp. tengcongensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 with 5 mM methyl syringate as substrate only traces, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.0004
-
 with 1 mM p-nitrophenyl palmitate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.03
-
 with 5 mM methyl vanillate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.05
-
 with 5 mM methyl caffeate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.426
-
 with 5 mM methyl p-coumarate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.456
-
 with 5 mM methyl ferulate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.462
-
 with feruloylated oligosaccharides as substrate, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.511
-
 with 5 mM methyl sinapate as substrate, at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
1.7
-
 with 1 mM p-nitrophenyl myristate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
10.5
-
 with 1 mM p-nitrophenyl caprylate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
18.5
-
 with 1 mM p-nitrophenyl acetate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
36
-
 with 1 mM p-nitrophenyl butyrate as substrate, at 25°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
feruloylated oligosaccharide + H2O ferulic acid is released from feruloylated oligosaccharides prepared from triticale bran. Recovery of 68 mg ferulic acid/100 g triticale bran by a 30% release of the total ferulic acid from triticale bran within a 5 h incubation period. Trichoderma viride xylanase combined with FAE can release 29.2 mg/100 g material Caldanaerobacter subterraneus subsp. tengcongensis ferulic acid + oligosaccharide
-
 ?
methyl caffeate + H2O poor substrate Caldanaerobacter subterraneus subsp. tengcongensis caffeic acid + methanol
-
 ?
methyl caffeate + H2O poor substrate Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 caffeic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis ferulic acid + methanol
-
 ?
methyl ferulate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 ferulic acid + methanol
-
 ?
methyl p-coumarate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-coumaric acid + methanol
-
 ?
methyl sinapate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis sinapic acid + methanol
-
 ?
methyl syringate + H2O poor substrate Caldanaerobacter subterraneus subsp. tengcongensis ? + methanol
-
 ?
methyl vanillate + H2O poor substrate Caldanaerobacter subterraneus subsp. tengcongensis ? + methanol
-
 ?
p-nitrophenyl acetate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + acetic acid
-
 ?
p-nitrophenyl acetate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 p-nitrophenol + acetic acid
-
 ?
p-nitrophenyl butyrate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + butyric acid
-
 ?
p-nitrophenyl butyrate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 p-nitrophenol + butyric acid
-
 ?
p-nitrophenyl caprylate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + caprylate
-
 ?
p-nitrophenyl myristate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + myristate
-
 ?
p-nitrophenyl palmitate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis p-nitrophenol + palmitate
-
 ?
p-nitrophenyl palmitate + H2O
-
 Caldanaerobacter subterraneus subsp. tengcongensis DSM 15242 / JCM 11007 / NBRC 100824 / MB4 p-nitrophenol + palmitate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE, purified enzyme Caldanaerobacter subterraneus subsp. tengcongensis

Synonyms

Synonyms Comment Organism
FAE
-
 Caldanaerobacter subterraneus subsp. tengcongensis
feruloyl esterase
-
 Caldanaerobacter subterraneus subsp. tengcongensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
75 80 at 75°C retains at least 95% of its original activity for over 80 min. At 80°C, its half-life is 50 min, rendering FAE a highly thermostable protein Caldanaerobacter subterraneus subsp. tengcongensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.22
-
 methyl p-coumarate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.28
-
 methyl ferulate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis
0.28
-
 methyl sinapate at 65°C, in 50 mM sodium phosphate buffer, pH 8.0 Caldanaerobacter subterraneus subsp. tengcongensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 purified enzyme, in 50 mM Britton and Robinson's universal buffer Caldanaerobacter subterraneus subsp. tengcongensis