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Literature summary for 3.1.1.73 extracted from
- Feruloyl esterase activity of the Clostridium thermocellum cellulosome can be attributed to previously unknown domains of XynY and XynZ (2000), J. Bacteriol., 182, 1346-1351.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| domains XynY and XynZ containing homolgy sequences to Orpinomyces feruloyl esterase cloned and expressed in Escherichia coli | Acetivibrio thermocellus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
domain XynZ, expected product of the 1,203-bp gene | Acetivibrio thermocellus |
| 2000000 | - |
cellulosome enzyme-complex | Acetivibrio thermocellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coastal bermuda grass + H2O | Acetivibrio thermocellus | - |
ferulic acid + ? | - |
? |  |
| feruloyl-polysaccharide + H2O | Aspergillus niger | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Pseudomonas fluorescens | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Butyrivibrio fibrisolvens | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Orpinomyces sp. | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Aspergillus tubingensis | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Acetivibrio thermocellus | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Neocallimastix sp. | - |
ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | Ruminococcus sp. | - |
ferulate + polysaccharide | - |
? | |
| wheat bran + H2O | Aspergillus niger | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
| wheat bran + H2O | Acetivibrio thermocellus | - |
ferulic acid + p-coumaric acid + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
| Acetivibrio thermocellus JW20 | - |
- |
- |
| Aspergillus niger | - |
- |
- |
| Aspergillus tubingensis | - |
- |
- |
| Butyrivibrio fibrisolvens | - |
- |
- |
| Neocallimastix sp. | - |
N. patriciarum | - |
| no activity in Escherichia coli | - |
- |
- |
| Orpinomyces sp. | - |
- |
- |
| Pseudomonas fluorescens | - |
subsp. cellulosa | - |
| Ruminococcus sp. | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant protein | Acetivibrio thermocellus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.6 | - |
FAE-XynZ | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | - |
Acetivibrio thermocellus | ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAX3-I | Neocallimastix sp. | ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| 5-O-[(E)-feruloyl]-[O-beta-D-xylopyranosyl-(1-2)]-O-alpha-L-arabinofuranosyl-[1-3]-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | - |
Acetivibrio thermocellus JW20 | ferulic acid + O-beta-D-xylopyranosyl-(1-2)-O-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| coastal bermuda grass + H2O | - |
Acetivibrio thermocellus | ferulic acid + ? | - |
? | |
| ethyl ferulate + H2O | - |
Aspergillus niger | ferulic acid + ethanol | - |
? | |
| ethyl ferulate + H2O | - |
Acetivibrio thermocellus | ferulic acid + ethanol | - |
? | |
| ethyl ferulate + H2O | - |
Acetivibrio thermocellus JW20 | ferulic acid + ethanol | - |
? | |
| ethyl p-coumarate + H2O | - |
Acetivibrio thermocellus | p-coumaric acid + ethanol | - |
? | |
| ethyl p-coumarate + H2O | - |
Acetivibrio thermocellus JW20 | p-coumaric acid + ethanol | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus niger | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Pseudomonas fluorescens | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Butyrivibrio fibrisolvens | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Orpinomyces sp. | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Aspergillus tubingensis | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Acetivibrio thermocellus | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Neocallimastix sp. | ferulate + polysaccharide | - |
? | |
| feruloyl-polysaccharide + H2O | - |
Ruminococcus sp. | ferulate + polysaccharide | - |
? | |
| additional information | - |
Aspergillus niger | ? | - |
? | |
| additional information | - |
Pseudomonas fluorescens | ? | - |
? | |
| additional information | - |
Neocallimastix sp. | ? | - |
? | |
| additional information | enzyme lacks activity towards carboxymethylcellulose, avicel, p-nitrophenyl(pNP)-arabinopyranoside, pNP-glucopyranoside, pNP-xylopyranoside and pNP-acetate | Acetivibrio thermocellus | ? | - |
? | |
| additional information | enzyme lacks activity towards carboxymethylcellulose, avicel, p-nitrophenyl(pNP)-arabinopyranoside, pNP-glucopyranoside, pNP-xylopyranoside and pNP-acetate | Acetivibrio thermocellus JW20 | ? | - |
? | |
| O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Acetivibrio thermocellus | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Neocallimastix sp. | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| O-5-O-[(E)-feruloyl]-alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose + H2O | FAXX | Acetivibrio thermocellus JW20 | ferulic acid + alpha-L-arabinofuranosyl-(1-3)-O-beta-D-xylopyranosyl-(1-4)-D-xylopyranose | - |
? | |
| wheat bran + H2O | - |
Aspergillus niger | ferulic acid + p-coumaric acid + ? | - |
? | |
| wheat bran + H2O | - |
Acetivibrio thermocellus | ferulic acid + p-coumaric acid + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cellulosome multi-enzyme complex | - |
Acetivibrio thermocellus |
| cellulosome xylanase Z feruloyl esterase | - |
Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Acetivibrio thermocellus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
70 | recombinant enzyme | Acetivibrio thermocellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 90 | stable for 6h, loses 50% activity at 80°C within 3 h, loses all activity after 1 h at 90°C | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | recombinant enzyme | Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | - |
Acetivibrio thermocellus |
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