Application | Comment | Organism |
---|---|---|
additional information | AChE from the juvenile Colossoma macropomum brain can be used as an alternative biocomponent of organophosphorus and carbamate biosensors in routine pesticide screening in the environment | Colossoma macropomum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Carbaryl | - |
Colossoma macropomum | |
carbofuran | - |
Colossoma macropomum | |
chlorpyrifos | - |
Colossoma macropomum | |
dichlorvos | - |
Colossoma macropomum | |
additional information | diazinon and temephos do not inhibit the enzyme | Colossoma macropomum | |
tetraethyl diphosphate | - |
Colossoma macropomum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.434 | - |
acetylthiocholine | in 0.5 mM Tris-HCl buffer, pH 7.4, at 22°C | Colossoma macropomum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Colossoma macropomum | - |
tambaqui | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | juvenile brain | Colossoma macropomum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetylthiocholine + H2O | - |
Colossoma macropomum | thiocholine + acetate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AChE | - |
Colossoma macropomum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Colossoma macropomum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme retains approximately 70% of activity after incubation at 50°C for 30 min | Colossoma macropomum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Colossoma macropomum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.00004 | - |
in 0.5 mM Tris-HCl buffer, pH 7.4, at 22°C | Colossoma macropomum | dichlorvos | |
0.00092 | - |
in 0.5 mM Tris-HCl buffer, pH 7.4, at 22°C | Colossoma macropomum | carbofuran | |
0.0037 | - |
in 0.5 mM Tris-HCl buffer, pH 7.4, at 22°C | Colossoma macropomum | tetraethyl diphosphate | |
0.0076 | - |
in 0.5 mM Tris-HCl buffer, pH 7.4, at 22°C | Colossoma macropomum | chlorpyrifos | |
0.0338 | - |
in 0.5 mM Tris-HCl buffer, pH 7.4, at 22°C | Colossoma macropomum | Carbaryl |