Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is a promising biocatalyst for the production of semi-synthetic beta-lactam antibiotics, penicillins and cephalosporins | Acidovorax avenae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidovorax avenae | Q6YBS3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxy-D-phenylglycine methyl ester + H2O | - |
Acidovorax avenae | 4-hydroxy-D-phenylglycine + methanol | - |
? | |
amoxicillin + H2O | - |
Acidovorax avenae | D-4-hydroxyphenylglycine amide + 6-aminopenicillanic acid | - |
? | |
ampicillin + H2O | - |
Acidovorax avenae | (R)-2-phenylglycine + 6-aminopenicillanic acid | - |
? | |
cephalexin + H2O | - |
Acidovorax avenae | D-phenylglycine + 7-aminodesacetoxycephalosporanic acid | - |
? | |
D-phenylglycine methyl ester + H2O | - |
Acidovorax avenae | D-phenylglycine + methanol | - |
? | |
additional information | modeling of enzyme complexes with various substrates to determine substrate specificity | Acidovorax avenae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AEH | - |
Acidovorax avenae |
alpha-amino acid ester hydrolase | - |
Acidovorax avenae |
General Information | Comment | Organism |
---|---|---|
additional information | key amino acid residues in the active site are the catalytic triad residues Ser175, His341 and Asp308, residues of the oxyanion hole are Tyr83 and Tyr176, and of the carboxylate cluster are carboxylate groups of Asp209, Glu310 and Asp311. The optimal configuration of residues in the active site occurs with a negative net charge -1 in the carboxylate cluster. Molecular dynamics simulations, docking, and three-dimensional structure homology modeling using the structure of the Xanthomonas campestris enzyme, PDB ID 1MPX, overview | Acidovorax avenae |