BRENDA - Enzyme Database show
show all sequences of 3.1.1.41

Purification, characterization and partial amino acid sequences of a novel cephalosporin-C deacetylase from Bacillus subtilis

Takimoto, A.; Mitsushima, K.; Yagi, S.; Sonoyama, T.; J. Ferment. Bioeng. 77, 17-22 (1994)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetate
competitive; weak
Bacillus subtilis
Cu2+
-
Bacillus subtilis
deacetyl-7-aminocephalosporanic acid
weak noncompetitive
Bacillus subtilis
DFP
-
Bacillus subtilis
additional information
the weak product inhibitors do not prevent the completion of the deacetylation
Bacillus subtilis
N-tosyl-L-Lys chloromethyl ketone
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
p-nitrophenyl acetate
-
Bacillus subtilis
6.5
-
7-aminocephalosporanic acid
strain ATCC6633
Bacillus subtilis
7.9
-
7-aminocephalosporanic acid
strain SHS 0133, FERM BP-2755
Bacillus subtilis
11.1
-
cephalotin
-
Bacillus subtilis
11.5
-
cephaloglycin
-
Bacillus subtilis
13.9
-
cephapirin
-
Bacillus subtilis
24
-
cephalosporin C
-
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
8 * 35000, strain SHS 0133, SDS-PAGE
Bacillus subtilis
150000
-
strain ATCC6633
Bacillus subtilis
280000
-
strain SHS 0133, gel filtration
Bacillus subtilis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
; strain ATCC6633
-
Bacillus subtilis ATCC 6633
-
strain ATCC6633
-
Bacillus subtilis SHS 0133
-
-
-
Purification (Commentary)
Commentary
Organism
strain SHS 0133, FERM BP-2755
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
187.4
-
hydrolysis of p-nitrophenyl acetate
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-aminocephalosporanic acid + H2O
-
170673
Bacillus subtilis
acetate + deacetyl-7-aminocephalosporanic acid
-
-
-
?
7-aminocephalosporanic acid + H2O
-
170673
Bacillus subtilis ATCC 6633
acetate + deacetyl-7-aminocephalosporanic acid
-
-
-
?
7-aminocephalosporanic acid + H2O
-
170673
Bacillus subtilis SHS 0133
acetate + deacetyl-7-aminocephalosporanic acid
-
-
-
?
cephaloglycin + H2O
-
170673
Bacillus subtilis
acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
?
cephaloglycin + H2O
-
170673
Bacillus subtilis ATCC 6633
acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
?
cephaloglycin + H2O
-
170673
Bacillus subtilis SHS 0133
acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
?
cephalosporin C + H2O
-
170673
Bacillus subtilis
deacetylcephalosporin C + acetate
-
-
-
?
cephalothin + H2O
-
170673
Bacillus subtilis
acetate + deacetylcephalothin
-
-
-
?
cephalothin + H2O
-
170673
Bacillus subtilis ATCC 6633
acetate + deacetylcephalothin
-
-
-
?
cephalothin + H2O
-
170673
Bacillus subtilis SHS 0133
acetate + deacetylcephalothin
-
-
-
?
cephapirin + H2O
-
170673
Bacillus subtilis
acetate + deacetylcephapirin
-
-
-
?
cephapirin + H2O
-
170673
Bacillus subtilis ATCC 6633
acetate + deacetylcephapirin
-
-
-
?
cephapirin + H2O
-
170673
Bacillus subtilis SHS 0133
acetate + deacetylcephapirin
-
-
-
?
p-nitrophenyl acetate + H2O
-
170673
Bacillus subtilis
4-nitrophenol + acetate
-
-
-
?
p-nitrophenyl acetate + H2O
-
170673
Bacillus subtilis ATCC 6633
4-nitrophenol + acetate
-
-
-
?
p-nitrophenyl acetate + H2O
-
170673
Bacillus subtilis SHS 0133
4-nitrophenol + acetate
-
-
-
?
Subunits
Subunits
Commentary
Organism
octamer
8 * 35000, strain SHS 0133, SDS-PAGE
Bacillus subtilis
tetramer
strain ATCC6633
Bacillus subtilis
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
strain SHS 0133, FERM BP-2755
Bacillus subtilis
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
pH 7.0, stable for at least 1 month
Bacillus subtilis
60
-
pH 7.0, 30 min, stable up to
Bacillus subtilis
65
-
pH 7.0, 30 min, 70% loss of activity
Bacillus subtilis
70
-
pH 7.0, 30 min, complete loss of activity
Bacillus subtilis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
55
-
Cephalothin
-
Bacillus subtilis
80
-
cephapirin
-
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
-
Bacillus subtilis
8
-
strain ATCC6633
Bacillus subtilis
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
7
10
37°C, 30 min, stable
Bacillus subtilis
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetate
competitive; weak
Bacillus subtilis
Cu2+
-
Bacillus subtilis
deacetyl-7-aminocephalosporanic acid
weak noncompetitive
Bacillus subtilis
DFP
-
Bacillus subtilis
additional information
the weak product inhibitors do not prevent the completion of the deacetylation
Bacillus subtilis
N-tosyl-L-Lys chloromethyl ketone
-
Bacillus subtilis
Zn2+
-
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
p-nitrophenyl acetate
-
Bacillus subtilis
6.5
-
7-aminocephalosporanic acid
strain ATCC6633
Bacillus subtilis
7.9
-
7-aminocephalosporanic acid
strain SHS 0133, FERM BP-2755
Bacillus subtilis
11.1
-
cephalotin
-
Bacillus subtilis
11.5
-
cephaloglycin
-
Bacillus subtilis
13.9
-
cephapirin
-
Bacillus subtilis
24
-
cephalosporin C
-
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
8 * 35000, strain SHS 0133, SDS-PAGE
Bacillus subtilis
150000
-
strain ATCC6633
Bacillus subtilis
280000
-
strain SHS 0133, gel filtration
Bacillus subtilis
Purification (Commentary) (protein specific)
Commentary
Organism
strain SHS 0133, FERM BP-2755
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
187.4
-
hydrolysis of p-nitrophenyl acetate
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
7-aminocephalosporanic acid + H2O
-
170673
Bacillus subtilis
acetate + deacetyl-7-aminocephalosporanic acid
-
-
-
?
7-aminocephalosporanic acid + H2O
-
170673
Bacillus subtilis ATCC 6633
acetate + deacetyl-7-aminocephalosporanic acid
-
-
-
?
7-aminocephalosporanic acid + H2O
-
170673
Bacillus subtilis SHS 0133
acetate + deacetyl-7-aminocephalosporanic acid
-
-
-
?
cephaloglycin + H2O
-
170673
Bacillus subtilis
acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
?
cephaloglycin + H2O
-
170673
Bacillus subtilis ATCC 6633
acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
?
cephaloglycin + H2O
-
170673
Bacillus subtilis SHS 0133
acetate + (6R,7R)-7-{[(2R)-2-amino-2-phenylacetyl]amino}-3-(hydroxymethyl)-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
-
?
cephalosporin C + H2O
-
170673
Bacillus subtilis
deacetylcephalosporin C + acetate
-
-
-
?
cephalothin + H2O
-
170673
Bacillus subtilis
acetate + deacetylcephalothin
-
-
-
?
cephalothin + H2O
-
170673
Bacillus subtilis ATCC 6633
acetate + deacetylcephalothin
-
-
-
?
cephalothin + H2O
-
170673
Bacillus subtilis SHS 0133
acetate + deacetylcephalothin
-
-
-
?
cephapirin + H2O
-
170673
Bacillus subtilis
acetate + deacetylcephapirin
-
-
-
?
cephapirin + H2O
-
170673
Bacillus subtilis ATCC 6633
acetate + deacetylcephapirin
-
-
-
?
cephapirin + H2O
-
170673
Bacillus subtilis SHS 0133
acetate + deacetylcephapirin
-
-
-
?
p-nitrophenyl acetate + H2O
-
170673
Bacillus subtilis
4-nitrophenol + acetate
-
-
-
?
p-nitrophenyl acetate + H2O
-
170673
Bacillus subtilis ATCC 6633
4-nitrophenol + acetate
-
-
-
?
p-nitrophenyl acetate + H2O
-
170673
Bacillus subtilis SHS 0133
4-nitrophenol + acetate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
octamer
8 * 35000, strain SHS 0133, SDS-PAGE
Bacillus subtilis
tetramer
strain ATCC6633
Bacillus subtilis
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
55
-
strain SHS 0133, FERM BP-2755
Bacillus subtilis
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
30
-
pH 7.0, stable for at least 1 month
Bacillus subtilis
60
-
pH 7.0, 30 min, stable up to
Bacillus subtilis
65
-
pH 7.0, 30 min, 70% loss of activity
Bacillus subtilis
70
-
pH 7.0, 30 min, complete loss of activity
Bacillus subtilis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
55
-
Cephalothin
-
Bacillus subtilis
80
-
cephapirin
-
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
8.5
-
Bacillus subtilis
8
-
strain ATCC6633
Bacillus subtilis
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
7
10
37°C, 30 min, stable
Bacillus subtilis
Other publictions for EC 3.1.1.41
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
749261
Singh
Structural role of a conserve ...
Thermotoga maritima, Thermotoga maritima ATCC 43589, Thermotoga maritima DSM 3109
Proteins
85
694-708
2017
-
-
1
1
2
-
-
18
-
-
2
-
-
6
-
-
1
-
-
-
13
-
23
-
3
-
1
20
1
1
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
18
-
-
2
-
-
-
-
1
-
-
13
-
23
-
3
-
1
20
1
1
-
-
-
-
-
-
19
19
746975
Singh
Crystal structure of Thermoto ...
Thermotoga maritima, Thermotoga maritima ATCC 43589, Thermotoga maritima DSM 3109
Biochem. Biophys. Res. Commun.
476
63-68
2016
-
-
-
1
-
-
-
-
-
-
-
-
-
8
-
-
1
-
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749834
Ma
-
High-level expression of Ceph ...
Bacillus subtilis, Bacillus subtilis SIL3
Biochem. Eng. J.
114
183-190
2016
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
749837
Ma
-
One-pot enzymatic production ...
Pseudomonas sp. SE83
Biochem. Eng. J.
95
1-8
2015
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
752071
Tao
-
Efficient production of perac ...
Bacillus subtilis
Process Biochem.
50
2121-2127
2015
-
-
1
-
1
-
1
5
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
5
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
-
-
-
5
5
729073
Tian
A novel cephalosporin deacetyl ...
Bacillus subtilis, Bacillus subtilis CICC 20034
Appl. Microbiol. Biotechnol.
98
2081-2089
2014
-
-
1
-
-
-
15
-
-
-
2
2
-
2
-
-
1
-
-
-
6
-
13
1
1
1
3
-
1
1
1
-
-
-
-
-
-
1
-
-
-
-
-
15
-
-
-
-
2
2
-
-
-
1
-
-
6
-
13
1
1
1
3
-
1
1
1
-
-
1
1
-
-
-
730072
Wang
Double knockout of beta-lactam ...
Escherichia coli, Escherichia coli JM105
J. Biosci. Bioeng.
113
737-741
2012
-
-
-
-
1
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
730907
Levisson
Functional and structural char ...
Thermotoga maritima
Proteins
80
1545-1559
2012
-
-
1
1
-
-
2
-
1
2
-
2
-
1
-
-
1
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
1
-
-
-
-
1
-
1
-
-
-
2
1
-
1
2
-
2
-
-
-
1
-
-
-
-
4
1
1
-
-
-
1
-
-
-
-
2
2
-
-
-
747164
Hedge
The structural basis for the ...
Thermotoga maritima, Thermotoga maritima DSM 3109
Biochim. Biophys. Acta
1824
1024-1030
2012
-
-
1
-
1
-
-
10
-
-
-
-
-
4
-
-
1
-
-
-
-
-
15
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
10
-
-
-
-
-
-
-
1
-
-
-
-
15
-
1
-
-
-
1
-
-
-
-
-
-
-
10
10
677538
Martinez-Martinez
A colorimetric assay for the d ...
Bacillus pumilus, Bacillus pumilus CECT 5072
Anal. Biochem.
369
210-217
2007
-
1
1
-
-
-
-
2
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
671502
Takimoto
Batch production of deacetyl 7 ...
Bacillus subtilis, Bacillus subtilis SHS0133
Appl. Microbiol. Biotechnol.
65
263-267
2004
-
1
1
-
-
1
1
-
-
-
-
2
-
5
-
-
1
-
-
-
-
1
4
-
-
-
1
-
-
-
2
-
-
-
-
-
1
1
-
-
-
1
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
1
4
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
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Purification and characterizat ...
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Takimoto
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Purification, characterization ...
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Singh
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Abbott
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Abbott
Immobilization of a cephalospo ...
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Hinnem
Enzymatic hydrolysis of cephal ...
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Nuesch
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Abbott
Physical properties and kineti ...
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Abraham
Cephalosporin acetylesterase ( ...
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Fujisawa
New findings on cephalosporin ...
Acremonium chrysogenum
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