Cloned (Comment) | Organism |
---|---|
His-tagged enzyme is expressed in Escherichia coli | Geobacillus thermocatenulatus |
Crystallization (Comment) | Organism |
---|---|
vapour-diffusion method at 20°C. The purified BTL2 lipase shows a strong tendency to aggregate upon concentration, which prevents a reproducible crystallization. Aggregation studies using dynamic light-scattering analysis are performed to improve the purification and concentration of BTL2 lipase. Different chemical classes of additives are tested to manipulate the aggregation behaviour of BTL2 lipase with the aim of obtaining a monodisperse sample to use for crystallization | Geobacillus thermocatenulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Geobacillus thermocatenulatus |
Organic Solvent | Comment | Organism |
---|---|---|
2-propanol | for the concentrated lipase, the availability of 5% 2-propanol is sufficient to hold the lipase in monomeric form | Geobacillus thermocatenulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermocatenulatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus thermocatenulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl palmitate + H2O | - |
Geobacillus thermocatenulatus | 4-nitrophenol + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Geobacillus thermocatenulatus |
More | different chemical classes of additives are tested to manipulate the aggregation behaviour of BTL2 lipase with the aim of obtaining a monodisperse sample to use for crystallization. For the process of concentration of BTL2 lipase in monomeric form, 2-propanol and dodecyl dimethylamine-N-oxide are necessary | Geobacillus thermocatenulatus |
Synonyms | Comment | Organism |
---|---|---|
BTL2 lipase | - |
Geobacillus thermocatenulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Geobacillus thermocatenulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Geobacillus thermocatenulatus |