Cloned (Comment) | Organism |
---|---|
expression of LipA in Escherichia coli BL21-SI | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
thermostable lipase LipA | - |
Bacillus sp. (in: Bacteria) Tp10A.1 | - |
thermostable lipase LipA | - |
Purification (Comment) | Organism |
---|---|
gene lipA, recombinant LipA from Escherichia coli | Bacillus sp. (in: Bacteria) |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
triacylglycerol + H2O = diacylglycerol + a carboxylate | enzyme shows high enantioselectivity on racemic substrates | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(RS)-1-phenylethyl acetate + H2O | enzyme hydrolyzes mainly the R-enantiomer, temperature-dependent, the enantioselectivity decreases with increasing temperature | Bacillus sp. (in: Bacteria) | (R)-1-phenylethanol + acetate + (R)-1-phenylethyl acetate | - |
? | |
4-nitrophenyl butyrate + H2O | low activity | Bacillus sp. (in: Bacteria) | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | low activity | Bacillus sp. (in: Bacteria) Tp10A.1 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl caprate + H2O | best substrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + caprate | - |
? | |
4-nitrophenyl caprylate + H2O | best substrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl caprylate + H2O | best substrate | Bacillus sp. (in: Bacteria) Tp10A.1 | 4-nitrophenol + caprylate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Bacillus sp. (in: Bacteria) Tp10A.1 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl laurate + H2O | best substrate | Bacillus sp. (in: Bacteria) | 4-nitrophenol + laurate | - |
? | |
4-nitrophenyl myristate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + myristate | - |
? | |
4-nitrophenyl palmitate + H2O | low activity | Bacillus sp. (in: Bacteria) | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl palmitate + H2O | low activity | Bacillus sp. (in: Bacteria) Tp10A.1 | 4-nitrophenol + palmitate | - |
? | |
4-nitrophenyl stearate + H2O | - |
Bacillus sp. (in: Bacteria) | 4-nitrophenol + stearate | - |
? | |
methyl(RS)-2-methyldecanoate + H2O | enzyme hydrolyzes mainly the R-enantiomer, temperature-dependent, the enantioselectivity decreases with increasing temperature | Bacillus sp. (in: Bacteria) | (R)-2-methyldecanoic acid + methanol | - |
? | |
additional information | LipA has a preference for 4-nitrophenyl esters with longer fatty acid chains | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | LipA has a preference for 4-nitrophenyl esters with longer fatty acid chains | Bacillus sp. (in: Bacteria) Tp10A.1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lipase | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
recombinant enzyme | Bacillus sp. (in: Bacteria) |