Any feedback?
Literature summary for 3.1.1.117 extracted from
- Purification, characterization and mass spectrometric sequencing of a thermophilic glucuronoyl esterase from Sporotrichum thermophile (2009), FEMS Microbiol. Lett., 296, 178-184 .
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.3 | - |
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | pH 6.0, 50°C | Thermothelomyces thermophilus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Thermothelomyces thermophilus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
gel filtration | Thermothelomyces thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Thermothelomyces thermophilus | the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols | ? | - |
- |
|
| additional information | Thermothelomyces thermophilus ATCC 34628 | the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces thermophilus | - |
- |
- |
| Thermothelomyces thermophilus ATCC 34628 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | StGE1 contains a noncatalytic carbohydrate-binding module | Thermothelomyces thermophilus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 74fold from culture broth, by ultrafiltration, two different steps of anion exchange chromatography, again ultrafiltration, and two different steps of gel filtration, followed by hydrophobic interaction chromatography | Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O | - |
Thermothelomyces thermophilus | methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | - |
? | |
| 4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O | - |
Thermothelomyces thermophilus ATCC 34628 | methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | - |
? | |
| additional information | the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols | Thermothelomyces thermophilus | ? | - |
- |
|
| additional information | the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols | Thermothelomyces thermophilus ATCC 34628 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 58000, SDS-PAGE | Thermothelomyces thermophilus |
| More | StGE1 is a modular enzyme, peptide fragmentation fingerprint, and mass spectrometric analysis, amino acid sequence comparison | Thermothelomyces thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| glucuronoyl esterase | - |
Thermothelomyces thermophilus |
| StGE1 | - |
Thermothelomyces thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermothelomyces thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | activity range, profile overview | Thermothelomyces thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 55 | purified esterase, 50% activity is retained after 430 and 286 min at 50°C and 55°C, and over 90% of maximal activity at 45°C after 6 h, inactivation after 6 h at 60°C | Thermothelomyces thermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.808 | - |
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | pH 6.0, 50°C | Thermothelomyces thermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermothelomyces thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 9 | activity range, profile overview | Thermothelomyces thermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
purified esterase, 6 h, 50°C, over 90% activity remaining at pH 8.0, 40% activity at pH 5.0 and pH 10.0, 20% at pH 11.0, and 10% at pH 4.0, inactivation after 6 h at pH 3.0 | Thermothelomyces thermophilus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermothelomyces thermophilus | isoelectric focusing | - |
6.7 |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme StGE1 is a family 15 glucuronoyl esterase that shows the highest homology with the hypothetical glucuronoyl esterase CHGG_10774 (UniProt ID Q2GMN0) of Chaetomium globosum strain CBS 148.51 | Thermothelomyces thermophilus |
| physiological function | the enzyme hydrolyzes the ester linkage between the 4-O-methyl-D-glucuronic acid of glucuronoxylan and lignin alcohols | Thermothelomyces thermophilus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.622 | - |
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside | pH 6.0, 50°C | Thermothelomyces thermophilus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
