Literature summary for 3.1.1.117 extracted from

Duranova, M.; Spanikova, S.; Woesten, H.A.; Biely, P.; de Vries, R.P.
Two glucuronoyl esterases of Phanerochaete chrysosporium (2009), Arch. Microbiol., 191, 133-140 .

Search for more literature for 3.1.1.117

Cloned(Commentary)

Cloned (Comment)	Organism
gene ge1 or e_gwh2.18.77.1, sequence analysis of the promoter region of gene ge1 reveals several putative regulatory sites, a TATA box is located -62 bp and a CCAAT-box -72 bp, upstream of ge1 translation initial site, sequence comparisons and phylogenetic analysis, functional recombinant heterologous expression of the extracellular glucuronoyl esterase in Aspergillus niger, Aspergillus vadensis strain NW282 (under control of the glucoamylase glaA promoter), Pycnoporus cinnabarinus strain BMR44 (under control of the GPD promoter), and Schizophyllum commune strain 4.8B (under control of the SC3 promoter), the enzyme expressed in Schizophyllum commune is secreted to the culture medium, the enzyme expressed in Aspergillus niger is inactive	Phanerodontia chrysosporium
gene ge2 or fgenesh1_pg.C_scaffold_11000167, sequence analysis of the promoter region of gene ge2 reveals several putative regulatory sites, the ge2 promoter contains a TATA box at position -62, sequence comparisons and phylogenetic analysis, functional recombinant heterologous expression of the extracellular glucuronoyl esterase in Schizophyllum commune strain 4.8B, the enzyme is secreted to the culture medium	Phanerodontia chrysosporium

Cloned (Comment)

Organism

gene ge1 or e_gwh2.18.77.1, sequence analysis of the promoter region of gene ge1 reveals several putative regulatory sites, a TATA box is located -62 bp and a CCAAT-box -72 bp, upstream of ge1 translation initial site, sequence comparisons and phylogenetic analysis, functional recombinant heterologous expression of the extracellular glucuronoyl esterase in Aspergillus niger, Aspergillus vadensis strain NW282 (under control of the glucoamylase glaA promoter), Pycnoporus cinnabarinus strain BMR44 (under control of the GPD promoter), and Schizophyllum commune strain 4.8B (under control of the SC3 promoter), the enzyme expressed in Schizophyllum commune is secreted to the culture medium, the enzyme expressed in Aspergillus niger is inactive

Phanerodontia chrysosporium

gene ge2 or fgenesh1_pg.C_scaffold_11000167, sequence analysis of the promoter region of gene ge2 reveals several putative regulatory sites, the ge2 promoter contains a TATA box at position -62, sequence comparisons and phylogenetic analysis, functional recombinant heterologous expression of the extracellular glucuronoyl esterase in Schizophyllum commune strain 4.8B, the enzyme is secreted to the culture medium

Phanerodontia chrysosporium

Organism

Organism	UniProt	Comment	Textmining
Phanerodontia chrysosporium	P0CT87	Sporotrichum pruinosum	-
Phanerodontia chrysosporium	P0CT88	Sporotrichum pruinosum	-
Phanerodontia chrysosporium ATCC MYA-4764	P0CT87	Sporotrichum pruinosum	-
Phanerodontia chrysosporium ATCC MYA-4764	P0CT88	Sporotrichum pruinosum	-
Phanerodontia chrysosporium FGSC 9002	P0CT87	Sporotrichum pruinosum	-
Phanerodontia chrysosporium FGSC 9002	P0CT88	Sporotrichum pruinosum	-
Phanerodontia chrysosporium RP-78	P0CT87	Sporotrichum pruinosum	-
Phanerodontia chrysosporium RP-78	P0CT88	Sporotrichum pruinosum	-

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	recombinant enzyme expressed in Aspergillus vadensis strain NW282 shows 0.036 U/ml activity, the enzyme expressed from Pycnoporus cinnabarinus strain BMR44 shows 0.417 U/ml, and the enzyme from Schizophyllum commune strain 4.8B 0.453 U/ml, at pH 6.0, 30°C	Phanerodontia chrysosporium
additional information	-	recombinant enzyme expressed in Schizophyllum commune strain 4.8B shows 0.530 U/ml activity, at pH 6.0, 30°C	Phanerodontia chrysosporium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
4-nitrophenyl-2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Phanerodontia chrysosporium	?	-	?
4-nitrophenyl-2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Phanerodontia chrysosporium ATCC MYA-4764	?	-	?
4-nitrophenyl-2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Phanerodontia chrysosporium FGSC 9002	?	-	?
4-nitrophenyl-2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O	-	Phanerodontia chrysosporium RP-78	?	-	?
methyl-4-O-methyl-D-glucuronate + H2O	-	Phanerodontia chrysosporium	methanol + 4-O-methyl-D-glucuronic acid	-	?
methyl-4-O-methyl-D-glucuronate + H2O	-	Phanerodontia chrysosporium ATCC MYA-4764	methanol + 4-O-methyl-D-glucuronic acid	-	?
methyl-4-O-methyl-D-glucuronate + H2O	-	Phanerodontia chrysosporium FGSC 9002	methanol + 4-O-methyl-D-glucuronic acid	-	?
methyl-4-O-methyl-D-glucuronate + H2O	-	Phanerodontia chrysosporium RP-78	methanol + 4-O-methyl-D-glucuronic acid	-	?
additional information	the substrate and the reaction product 4-O-methyl-D-glucuronic acid are visualized with N-(1-naphthyl)ethylenediamine dihydrochloride reagent	Phanerodontia chrysosporium	?	-	-
additional information	the substrate and the reaction product 4-O-methyl-D-glucuronic acid are visualized with N-(1-naphthyl)ethylenediamine dihydrochloride reagent	Phanerodontia chrysosporium ATCC MYA-4764	?	-	-
additional information	the substrate and the reaction product 4-O-methyl-D-glucuronic acid are visualized with N-(1-naphthyl)ethylenediamine dihydrochloride reagent	Phanerodontia chrysosporium FGSC 9002	?	-	-
additional information	the substrate and the reaction product 4-O-methyl-D-glucuronic acid are visualized with N-(1-naphthyl)ethylenediamine dihydrochloride reagent	Phanerodontia chrysosporium RP-78	?	-	-

Synonyms

Synonyms	Comment	Organism
4-O-methyl-glucuronoyl methylesterase 1	-	Phanerodontia chrysosporium
4-O-methyl-glucuronoyl methylesterase 2	-	Phanerodontia chrysosporium
GE1	-	Phanerodontia chrysosporium
Ge2	-	Phanerodontia chrysosporium
glucuronoyl esterase	-	Phanerodontia chrysosporium
glucuronoyl methylesterase 1	-	Phanerodontia chrysosporium
glucuronoyl methylesterase 2	-	Phanerodontia chrysosporium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Phanerodontia chrysosporium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Phanerodontia chrysosporium

General Information

General Information	Comment	Organism
evolution	the amino acid sequence of GE1 is used to identify homologous genes in the genomes of twenty-four fungi. Approximately half of the genomes, both from ascomycetes and basidiomycetes, contain putative orthologues, but their presence cannot be assigned to any of fungal class or subclass. Comparison of the amino acid sequences of identified and putative glucuronoyl esterases to other types of carbohydrate esterases (CE) confirms that they form a separate family of CEs	Phanerodontia chrysosporium