Literature summary for 3.1.1.117 extracted from

Wood, S.J.; Li, X.L.; Cotta, M.A.; Biely, P.; Duke, N.E.; Schiffer, M.; Pokkuluri, P.R.
Crystallization and preliminary X-ray diffraction analysis of the glucuronoyl esterase catalytic domain from Hypocrea jecorina (2008), Acta Crystallogr. Sect. F, 64, 255-257 .

Search for more literature for 3.1.1.117

Cloned(Commentary)

Cloned (Comment)	Organism
gene cip2, recombinant expression of the isolated C-terminal domain (corresponding to amino acids 90-460) in Hypocrea jecorina cells, the protein is secreted into culture medium	Trichoderma reesei

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified isolated glucuronoyl esterase catalytic domain, sitting drop vapor diffusion method using 1.4 M sodium/potassium phosphate pH 6.9, equilibrating a mixture of 400 nl 25 mg/ml protein in 20 mM Tris, pH 7.5, with 400 nl screen solution over a reservoir of 0.2 ml screen solution, containing 27% w/v sucrose and 0.5 M NaBr for crystal 1, 27% w/v sucrose and 1.0 M NaBr for crystal 2, and 27% w/v sucrose only for crystal 3, the crystals belong to space group P212121, at 35°C, crystals typically form in 2-3 days and grow to full size in a week, X-ray diffraction structure determination and analysis at 1.95-2.50 A resolution	Trichoderma reesei

Crystallization (Comment)

Organism

purified isolated glucuronoyl esterase catalytic domain, sitting drop vapor diffusion method using 1.4 M sodium/potassium phosphate pH 6.9, equilibrating a mixture of 400 nl 25 mg/ml protein in 20 mM Tris, pH 7.5, with 400 nl screen solution over a reservoir of 0.2 ml screen solution, containing 27% w/v sucrose and 0.5 M NaBr for crystal 1, 27% w/v sucrose and 1.0 M NaBr for crystal 2, and 27% w/v sucrose only for crystal 3, the crystals belong to space group P212121, at 35°C, crystals typically form in 2-3 days and grow to full size in a week, X-ray diffraction structure determination and analysis at 1.95-2.50 A resolution

Trichoderma reesei

Organism

Organism	UniProt	Comment	Textmining
Trichoderma reesei	G0RV93	the sexual teleomorph of Trichoderma reese	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	Cip2 contains a carbohydrate-binding module	Trichoderma reesei

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl-4-O-methyl-D-glucuronate + H2O	-	Trichoderma reesei	methanol + 4-O-methyl-D-glucuronate	-	?

Subunits

Subunits	Comment	Organism
More	the full-length Cip2 protein contains a carbohydrate-binding module, a linker and a catalytic domain	Trichoderma reesei

Synonyms

Synonyms	Comment	Organism
Cip2	-	Trichoderma reesei
glucuronoyl esterase	-	Trichoderma reesei

General Information

General Information	Comment	Organism
evolution	the enzyme is a member of the carbohydrate esterase family 15 (CE15)	Trichoderma reesei