Literature summary for 3.1.1.117 extracted from

Sunner, H.; Charavgi, M.; Olsson, L.; Topakas, E.; Christakopoulos, P.
Glucuronoyl esterase screening and characterization assays utilizing commercially available benzyl glucuronic acid ester (2015), Molecules, 20, 17807-17817 .

Search for more literature for 3.1.1.117

Application

Application	Comment	Organism
biofuel production	broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials	Thermothelomyces thermophilus
biofuel production	broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials	Trichoderma reesei
biofuel production	broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials	Podospora anserina
biofuel production	broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials	Schizophyllum commune
biofuel production	broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials	Cerrena unicolor
biofuel production	broad range of industrial applications for the biocatalytic processes of lignocellulose degradation and modification of lignocellulosic materials	Phanerodontia chrysosporium
molecular biology	the enzyme may prove a valuable as research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry	Trichoderma reesei
molecular biology	the enzyme may prove a valuable as research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry	Schizophyllum commune
molecular biology	the enzyme may prove a valuable research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry	Phanerodontia chrysosporium
molecular biology	the enzyme may prove valuable as a research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry	Thermothelomyces thermophilus
molecular biology	the enzyme may prove valuable as a research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry	Podospora anserina
molecular biology	the enzyme may prove valuable as a research tool for the investigation of lignin and lignin to carbohydrates-bond chemistry	Cerrena unicolor

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism
additional information	-	additional information	Michaelis-Menten kinetics	Thermothelomyces thermophilus
additional information	-	additional information	Michaelis-Menten kinetics	Podospora anserina
8.9	-	1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol	pH 6.0, 45°C	Thermothelomyces thermophilus
12.1	-	1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol	pH 6.0, 35°C	Podospora anserina

Organism

Organism	UniProt	Comment	Textmining
Cerrena unicolor	A0A0A7EQR3	-	-
Phanerodontia chrysosporium	P0CT87	-	-
Phanerodontia chrysosporium	P0CT88	-	-
Phanerodontia chrysosporium RP-78	P0CT87	-	-
Podospora anserina	B2ABS0	-	-
Podospora anserina	B2ABS0	Pleurage anserina	-
Podospora anserina ATCC MYA-4624	B2ABS0	Pleurage anserina	-
Podospora anserina DSM 980	B2ABS0	-	-
Podospora anserina DSM 980	B2ABS0	Pleurage anserina	-
Podospora anserina FGSC 10383	B2ABS0	Pleurage anserina	-
Podospora anserina S	B2ABS0	Pleurage anserina	-
Schizophyllum commune	D8QLP9	-	-
Schizophyllum commune H4-8	D8QLP9	-	-
Thermothelomyces thermophilus	G2QJR6	-	-
Thermothelomyces thermophilus	G2QJR6	Sporotrichum thermophile	-
Thermothelomyces thermophilus ATCC 42464	G2QJR6	Sporotrichum thermophile	-
Thermothelomyces thermophilus BCRC 31852	G2QJR6	Sporotrichum thermophile	-
Thermothelomyces thermophilus DSM 1799	G2QJR6	-	-
Thermothelomyces thermophilus DSM 1799	G2QJR6	Sporotrichum thermophile	-
Trichoderma reesei	G0RV93	-	-
Trichoderma reesei QM6a	G0RV93	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Thermothelomyces thermophilus	?	-	?
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Podospora anserina	?	-	?
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Thermothelomyces thermophilus ATCC 42464	?	-	?
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Podospora anserina ATCC MYA-4624	?	-	?
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Podospora anserina DSM 980	?	-	?
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Podospora anserina FGSC 10383	?	-	?
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Thermothelomyces thermophilus BCRC 31852	?	-	?
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Podospora anserina S	?	-	?
1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol + H2O	-	Thermothelomyces thermophilus DSM 1799	?	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Trichoderma reesei	?	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Phanerodontia chrysosporium	?	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Trichoderma reesei QM6a	?	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Thermothelomyces thermophilus	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Podospora anserina	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Cerrena unicolor	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Phanerodontia chrysosporium	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Thermothelomyces thermophilus ATCC 42464	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Podospora anserina ATCC MYA-4624	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Podospora anserina DSM 980	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Podospora anserina FGSC 10383	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Thermothelomyces thermophilus BCRC 31852	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Podospora anserina S	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Thermothelomyces thermophilus DSM 1799	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Phanerodontia chrysosporium RP-78	benzyl alcohol + D-glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Schizophyllum commune	benzyl alcohol + glucuronic acid	-	?
benzyl D-glucuronic acid ester + H2O	the application of this substrate is described (a) in a Thin Layer Chromatography (TLC) assay for qualitative activity assessment (b) a spectrophotometer-based assay useful for screening, and (c) an HPLC-based assay allowing more precise activity determinations, enabling enzyme kinetics characterization. In addition, TLC and spectrophotometric methods are less costly and time consuming, and there is no need for complicated instruments for the analysis	Schizophyllum commune H4-8	benzyl alcohol + glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Thermothelomyces thermophilus	benzyl alcohol + D-glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Podospora anserina	benzyl alcohol + D-glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Thermothelomyces thermophilus ATCC 42464	benzyl alcohol + D-glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Podospora anserina ATCC MYA-4624	benzyl alcohol + D-glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Podospora anserina DSM 980	benzyl alcohol + D-glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Podospora anserina FGSC 10383	benzyl alcohol + D-glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Thermothelomyces thermophilus BCRC 31852	benzyl alcohol + D-glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Podospora anserina S	benzyl alcohol + D-glucuronic acid	-	?
benzyl-D-glucuronate + H2O	-	Thermothelomyces thermophilus DSM 1799	benzyl alcohol + D-glucuronic acid	-	?
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Thermothelomyces thermophilus	?	-	-
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Podospora anserina	?	-	-
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Thermothelomyces thermophilus ATCC 42464	?	-	-
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Podospora anserina ATCC MYA-4624	?	-	-
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Podospora anserina DSM 980	?	-	-
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Podospora anserina FGSC 10383	?	-	-
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Thermothelomyces thermophilus BCRC 31852	?	-	-
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Podospora anserina S	?	-	-
additional information	design of BnGlcA-based glucuronoyl esterase (GE) assays for different applications: a thin-layer chromatography assay for qualitative activity detection, a coupled-enzyme spectrophotometric assay that can be used for high-throughput screening or general activity determinations and a HPLC-based detection method allowing kinetic determinations. The three-level experimental procedure not merely facilitates routine, fast and simple biochemical characterizations but it can also give rise to the discovery different GEs through an extensive screening of heterologous genomic and metagenomic expression libraries	Thermothelomyces thermophilus DSM 1799	?	-	-

Synonyms

Synonyms	Comment	Organism
4-O-methyl-glucuronoyl methylesterase	UniProt	Thermothelomyces thermophilus
4-O-methyl-glucuronoyl methylesterase	UniProt	Podospora anserina
CuGE	-	Cerrena unicolor
GE Cip2	-	Trichoderma reesei
GE1	-	Podospora anserina
Ge2	-	Thermothelomyces thermophilus
glucuronoyl esterase	-	Thermothelomyces thermophilus
glucuronoyl esterase	-	Podospora anserina
glucuronoyl esterase 1	-	Podospora anserina
glucuronoyl esterase 2	-	Thermothelomyces thermophilus
PaGE1	-	Podospora anserina
PcGE1	-	Phanerodontia chrysosporium
PcGE2	-	Phanerodontia chrysosporium
ScGE1	-	Schizophyllum commune
StGE2	-	Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	assay at	Podospora anserina
45	-	assay at	Thermothelomyces thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.5	-	1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol	pH 6.0, 45°C	Thermothelomyces thermophilus
7.8	-	1-(4-hydroxy-3-methoxyphenyl)-1-(alpha-D-glucuronate)-2-(2-methoxyphenoxy)-3-propanol	pH 6.0, 35°C	Podospora anserina

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Thermothelomyces thermophilus
6	-	assay at	Podospora anserina