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Literature summary for 3.1.1.117 extracted from

  • dErrico, C.; Börjesson, J.; Ding, H.; Krogh, K.; Spodsberg, N.; Madsen, R.; Monrad, R.
    Improved biomass degradation using fungal glucuronoyl-esterases-hydrolysis of natural corn fiber substrate (2016), J. Biotechnol., 219, 117-123 .
    View publication on PubMed
Search for more literature for 3.1.1.117

Application

Application Comment Organism
biofuel production inclusion of glucuronoyl-esterases in the enzymatic digestion of lignocellulosic biomass Trichoderma reesei
biofuel production inclusion of glucuronoyl-esterases in the enzymatic digestion of lignocellulosic biomass Schizophyllum commune
biofuel production inclusion of glucuronoyl-esterases in the enzymatic digestion of lignocellulosic biomass Cerrena unicolor

Cloned(Commentary)

Cloned (Comment) Organism
cloned into the expression vector pDAU109 and recombinantly expressed in the host Aspergillus oryzae MT3568 Trichoderma reesei
cloned into the expression vector pDAU109 and recombinantly expressed in the host Aspergillus oryzae MT3568 Schizophyllum commune
cloned into the expression vector pDAU109 and recombinantly expressed in the host Aspergillus oryzae MT3568 Cerrena unicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 4-O-methyl-glucuronic acid gamma-linked to a lignin dimer pH 6.0, 30°C Schizophyllum commune
3.4
-
 4-O-methyl-glucuronic acid gamma-linked to a lignin dimer pH 6.0, 30°C Cerrena unicolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 SDS-PAGE Trichoderma reesei

Organism

Organism UniProt Comment Textmining
Cerrena unicolor A0A0A7EQR3
-
-
Schizophyllum commune D8QLP9
-
-
Schizophyllum commune H4-8 D8QLP9
-
-
Trichoderma reesei G0RV93 i.e. Trichoderma reesei
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trichoderma reesei

Purification (Commentary)

Purification (Comment) Organism
-
 Trichoderma reesei
-
 Schizophyllum commune
-
 Cerrena unicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-glucuronic acid gamma-linked to a lignin dimer + H2O
-
 Schizophyllum commune ?
-
 ?
4-O-methyl-glucuronic acid gamma-linked to a lignin dimer + H2O
-
 Cerrena unicolor ?
-
 ?
4-O-methyl-glucuronic acid gamma-linked to a lignin dimer + H2O
-
 Schizophyllum commune H4-8 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Trichoderma reesei

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Trichoderma reesei
30
-
 assay at Schizophyllum commune
30
-
 assay at Cerrena unicolor

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
71
-
 thermal denaturation temperature Trichoderma reesei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
125
-
 4-O-methyl-glucuronic acid gamma-linked to a lignin dimer pH 6.0, 30°C Schizophyllum commune
285
-
 4-O-methyl-glucuronic acid gamma-linked to a lignin dimer pH 6.0, 30°C Cerrena unicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Trichoderma reesei
6
-
 assay at Schizophyllum commune
6
-
 assay at Cerrena unicolor

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 good stability at pH 5.0 Trichoderma reesei

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
83
-
 4-O-methyl-glucuronic acid gamma-linked to a lignin dimer pH 6.0, 30°C Cerrena unicolor
89
-
 4-O-methyl-glucuronic acid gamma-linked to a lignin dimer pH 6.0, 30°C Schizophyllum commune