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Literature summary for 3.1.1.117 extracted from

  • Topakas, E.; Moukouli, M.; Dimarogona, M.; Vafiadi, C.; Christakopoulos, P.
    Functional expression of a thermophilic glucuronyl esterase from Sporotrichum thermophile identification of the nucleophilic serine (2010), Appl. Microbiol. Biotechnol., 87, 1765-1772 .
    View publication on PubMed
Search for more literature for 3.1.1.117

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Thermothelomyces thermophilus
gene ge2, DNA and amino acid sequence determination and analysis, sequence comparisons, gene ge2 from the genomic DNA is successfully cloned in frame with the sequence for the Saccharomyces cerevisiae alpha-factor secretion signal under the transcriptional control of alcohol oxidase (AOX1) promoter and integrated in Pichia pastoris strain X-33 Thermothelomyces thermophilus

Protein Variants

Protein Variants Comment Organism
S213A complete loss of activity Thermothelomyces thermophilus
S213A site-directed mutagenesis Thermothelomyces thermophilus

Inhibitors

Inhibitors Comment Organism Structure
PMSF 10 mM, 10 min, 60% loss of activity Thermothelomyces thermophilus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Thermothelomyces thermophilus
-
-

Organism

Organism UniProt Comment Textmining
Thermothelomyces thermophilus G2QJR6
-
-
Thermothelomyces thermophilus G2QJR6 Sporotrichum thermophile
-
Thermothelomyces thermophilus ATCC 42464 G2QJR6 Sporotrichum thermophile
-
Thermothelomyces thermophilus BCRC 31852 G2QJR6 Sporotrichum thermophile
-
Thermothelomyces thermophilus DSM 1799 G2QJR6
-
-
Thermothelomyces thermophilus DSM 1799 G2QJR6 Sporotrichum thermophile
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Pichia pastoris strain X-33 by ultrafiltration, dialysis, immobilized metal-ion affinity chromatography, and again ultrafiltration and dialysis Thermothelomyces thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Thermothelomyces thermophilus methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside
-
 ?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Thermothelomyces thermophilus ATCC 42464 methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside
-
 ?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Thermothelomyces thermophilus BCRC 31852 methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside
-
 ?
4-nitrophenyl 2-O-(methyl-4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside + H2O
-
 Thermothelomyces thermophilus DSM 1799 methanol + 4-nitrophenyl 2-O-(4-O-methyl-alpha-D-glucopyranosyluronate)-beta-D-xylopyranoside
-
 ?
methyl 4-O-methyl-alpha-D-glucopyranuronate + H2O
-
 Thermothelomyces thermophilus methanol + 4-O-methyl-alpha-D-glucopyranuronate
-
 ?
methyl 4-O-methyl-alpha-D-glucopyranuronate + H2O
-
 Thermothelomyces thermophilus ATCC 42464 methanol + 4-O-methyl-alpha-D-glucopyranuronate
-
 ?
methyl 4-O-methyl-alpha-D-glucopyranuronate + H2O
-
 Thermothelomyces thermophilus BCRC 31852 methanol + 4-O-methyl-alpha-D-glucopyranuronate
-
 ?
methyl 4-O-methyl-alpha-D-glucopyranuronate + H2O
-
 Thermothelomyces thermophilus DSM 1799 methanol + 4-O-methyl-alpha-D-glucopyranuronate
-
 ?
methyl 4-O-methyl-D-glucopyranuronate + H2O
-
 Thermothelomyces thermophilus methanol + 4-O-methyl-D-glucopyranuronate
-
 ?
methyl 4-O-methyl-D-glucopyranuronate + H2O
-
 Thermothelomyces thermophilus ATCC 42464 methanol + 4-O-methyl-D-glucopyranuronate
-
 ?
methyl 4-O-methyl-D-glucopyranuronate + H2O
-
 Thermothelomyces thermophilus BCRC 31852 methanol + 4-O-methyl-D-glucopyranuronate
-
 ?
methyl 4-O-methyl-D-glucopyranuronate + H2O
-
 Thermothelomyces thermophilus DSM 1799 methanol + 4-O-methyl-D-glucopyranuronate
-
 ?
additional information enzyme is active on substrates containing glucuronic acid methyl ester Thermothelomyces thermophilus ?
-
 ?
additional information the enzyme is active on substrates containing glucuronic acid methyl ester Thermothelomyces thermophilus ?
-
-
additional information enzyme is active on substrates containing glucuronic acid methyl ester Thermothelomyces thermophilus ATCC 42464 ?
-
 ?
additional information the enzyme is active on substrates containing glucuronic acid methyl ester Thermothelomyces thermophilus ATCC 42464 ?
-
-
additional information enzyme is active on substrates containing glucuronic acid methyl ester Thermothelomyces thermophilus BCRC 31852 ?
-
 ?
additional information the enzyme is active on substrates containing glucuronic acid methyl ester Thermothelomyces thermophilus BCRC 31852 ?
-
-
additional information enzyme is active on substrates containing glucuronic acid methyl ester Thermothelomyces thermophilus DSM 1799 ?
-
 ?
additional information the enzyme is active on substrates containing glucuronic acid methyl ester Thermothelomyces thermophilus DSM 1799 ?
-
-

Subunits

Subunits Comment Organism
? x * 42881, calulated, x * 43000, recombinant protein with His-tag Thermothelomyces thermophilus
? x * 43000, recombinant enzyme, SDS-PAGE, x * 40056, sequence calculation Thermothelomyces thermophilus

Synonyms

Synonyms Comment Organism
4-O-methyl-glucuronoyl methylesterase UniProt Thermothelomyces thermophilus
Ge2
-
 Thermothelomyces thermophilus
glucuronoyl esterase
-
 Thermothelomyces thermophilus
glucuronoyl esterase 2
-
 Thermothelomyces thermophilus
StGE2
-
 Thermothelomyces thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Thermothelomyces thermophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 over 60% of maximal activity within this range, profile overview Thermothelomyces thermophilus
60 70 more than 70% of maximum activity Thermothelomyces thermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Thermothelomyces thermophilus
50
-
 recombinant purified enzyme, pH 7.0, stable up to for 24 h Thermothelomyces thermophilus
55
-
 pH 7.0, 24 h, 53% loss of activity Thermothelomyces thermophilus
55 60 recombinant purified enzyme, pH 7.0, 55°C for 24 h, loss of 53% activity, inactivation above 60°C, half-lives are 22.5 h and 0.5 h at 55°C and 60°C, respectively Thermothelomyces thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Thermothelomyces thermophilus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 over 70% of maximal activity within this range, profile overview Thermothelomyces thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10
-
 Thermothelomyces thermophilus
4 10 recombinant purified esterase displays broad pH range stability between 4-10 Thermothelomyces thermophilus
7
-
 55°C, 24 h, 53% loss of activity Thermothelomyces thermophilus

pI Value

Organism Comment pI Value Maximum pI Value
Thermothelomyces thermophilus sequence calculation
-
 5.76

General Information

General Information Comment Organism
evolution the enzyme belongs to the carbohydrate esterase family 15 (CE-15) Thermothelomyces thermophilus
additional information the consensus sequence G-C-S-R-X-G features the characteristic serine residue involved in the generally conserved catalytic mechanism of the esterase family, the candidate nucleophilic residue Ser213 is responsible for catalyzing the enzymatic reaction Thermothelomyces thermophilus
physiological function the consensus sequence G-C-S-R-X-G features the characteristic serine residue involved in the generally conserved catalytic mechanism of the esterase family Thermothelomyces thermophilus