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Literature summary for 3.1.1.107 extracted from
- Structural characterization of enterobactin hydrolase IroE (2006), Biochemistry, 45, 10184-10190 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Escherichia coli |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| native enzyme and enzyme in complex with diisopropyl fluorophosphonate, sitting drop vapor diffusion method, using 6-10% (w/v) PEG 3350 and 100 mM HEPES (pH 7.5) | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D250A | the mutant shows 59% activity with enterobactin and 72% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
| D250N | the mutant shows 72% activity with enterobactin and 62% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
| D90A | the mutant shows 0.4% activity with enterobactin and 0.56% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
| D90N | the mutant shows 1.5% activity with enterobactin and 1.5% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
| E253A | the mutant shows 78% activity with enterobactin and 86% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
| E253Q | the mutant shows 92% activity with enterobactin and 97% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
| H287A | inactive | Escherichia coli |
| R130K | the mutant shows 0.036% activity with enterobactin and 0.41% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
| R130Q | inactive | Escherichia coli |
| S189A | inactive | Escherichia coli |
| S245D | the mutant shows 60% activity with enterobactin and 60% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
| S245E | the mutant shows 84% activity with enterobactin and 66% activity with diglucosyl-enterobactin compared to the wild type enzyme | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diisopropyl fluorophosphonate | - |
Escherichia coli |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Escherichia coli | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diglucosyl-enterobactin + H2O | Escherichia coli | - |
diglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| diglucosyl-enterobactin + H2O | Escherichia coli CFT073 | - |
diglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| enterobactin + H2O | Escherichia coli | - |
N-(2,3-dihydroxybenzoyl)-L-serine trimer | - |
? | |
| enterobactin + H2O | Escherichia coli CFT073 | - |
N-(2,3-dihydroxybenzoyl)-L-serine trimer | - |
? | |
| monoglucosyl-enterobactin + H2O | Escherichia coli | - |
monoglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| monoglucosyl-enterobactin + H2O | Escherichia coli CFT073 | - |
monoglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| salmochelin S4 + H2O | Escherichia coli | - |
salmochelin S2 | - |
? | |
| salmochelin S4 + H2O | Escherichia coli CFT073 | - |
salmochelin S2 | - |
? | |
| triglucosyl-enterobactin + H2O | Escherichia coli | - |
triglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| triglucosyl-enterobactin + H2O | Escherichia coli CFT073 | - |
triglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | A0A0H2V871 | - |
- |
| Escherichia coli CFT073 | A0A0H2V871 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Superdex S200 gel filtration | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diglucosyl-enterobactin + H2O | - |
Escherichia coli | diglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| diglucosyl-enterobactin + H2O | - |
Escherichia coli CFT073 | diglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| enterobactin + H2O | - |
Escherichia coli | N-(2,3-dihydroxybenzoyl)-L-serine trimer | - |
? | |
| enterobactin + H2O | - |
Escherichia coli CFT073 | N-(2,3-dihydroxybenzoyl)-L-serine trimer | - |
? | |
| monoglucosyl-enterobactin + H2O | - |
Escherichia coli | monoglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| monoglucosyl-enterobactin + H2O | - |
Escherichia coli CFT073 | monoglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| salmochelin S4 + H2O | - |
Escherichia coli | salmochelin S2 | - |
? | |
| salmochelin S4 + H2O | - |
Escherichia coli CFT073 | salmochelin S2 | - |
? | |
| triglucosyl-enterobactin + H2O | - |
Escherichia coli | triglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
| triglucosyl-enterobactin + H2O | - |
Escherichia coli CFT073 | triglucosyl-(2,3-dihydroxybenzoylserine)3 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 30800, calculated from amino acid sequence | Escherichia coli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| enterobactin hydrolase | - |
Escherichia coli |
| iroE | - |
Escherichia coli |
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