The 39-kDa poly(ADP-ribose) glycohydrolase ARH3 hydrolyzes O-acetyl-ADP-ribose, a product of the Sir2 family of acetyl-histone deacetylases

Ono, T.; Kasamatsu, A.; Oka, S.; Moss, J.; Proc. Natl. Acad. Sci. USA 103, 16687-16691 (2006)

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

ADP-D-ribose

competitive inhibitor

Homo sapiens

additional information

D-ribose 5-phosphate, AMP, ADP and beta-NAD have relatively little inhibitory effect on the enzyme activity

Homo sapiens

Metals/Ions

Commentary

Organism

Structure

Mg2+

dependent on Mg2+. 1 mM is used in assay conditions

Homo sapiens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

O-acetyl-ADP-D-ribose + H2O

Homo sapiens

-

ADP-D-ribose + acetate

-

-

?

poly(ADP-D-ribose) + H2O

Homo sapiens

-

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

O-acetyl-ADP-D-ribose + H2O

-

752026

Homo sapiens

ADP-D-ribose + acetate

-

-

-

?

poly(ADP-D-ribose) + H2O

-

752026

Homo sapiens

?

-

-

-

?

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

0.002

-

ADP-D-ribose

at pH 7.0 and 30°C

Homo sapiens

Inhibitors

Commentary

Organism

Structure

ADP-D-ribose

competitive inhibitor

Homo sapiens

additional information

D-ribose 5-phosphate, AMP, ADP and beta-NAD have relatively little inhibitory effect on the enzyme activity

Homo sapiens

Ki Value [mM]

Ki Value maximum [mM]

Inhibitor

Commentary

Organism

Structure

0.002

-

ADP-D-ribose

at pH 7.0 and 30°C

Homo sapiens

Metals/Ions

Commentary

Organism

Structure

Mg2+

dependent on Mg2+. 1 mM is used in assay conditions

Homo sapiens

Natural Substrates

Organism

Commentary (Nat. Sub.)

Natural Products

Commentary (Nat. Pro.)

Organism (Nat. Pro.)

Reversibility

ID

O-acetyl-ADP-D-ribose + H2O

Homo sapiens

-

ADP-D-ribose + acetate

-

-

?

poly(ADP-D-ribose) + H2O

Homo sapiens

-

?

-

-

?

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

O-acetyl-ADP-D-ribose + H2O

-

752026

Homo sapiens

ADP-D-ribose + acetate

-

-

-

?

poly(ADP-D-ribose) + H2O

-

752026

Homo sapiens

?

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

750645

Agnew

MacroD1 is a promiscuous ADP- ...

Homo sapiens, Mus musculus

Front. Microbiol.

9

20

2018

-

-

-

-

-

-

-

-

2

-

-

4

-

4

-

-

-

-

-

5

-

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8

2

4

-

-

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-

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-

-

-

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-

-

-

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2

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4

-

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-

-

5

-

-

8

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

752262

Haikarainen

Development of an inhibitor s ...

Homo sapiens

SLAS Discov.

23

255-263

2018

-

-

1

-

-

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5

-

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3

-

2

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1

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3

-

1

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4

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1

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4

5

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3

-

-

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1

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3

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-

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-

-

-

-

-

-

-

-

-

-

-

752189

Haikarainen

Proximal ADP-ribose hydrolysi ...

Trypanosoma brucei gambiense, Trypanosoma cruzi, Trypanosoma cruzi CL Brener

Sci. Rep.

6

24213

2016

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-

2

2

-

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9

-

8

-

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2

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9

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6

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2

-

2

-

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9

-

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-

2

-

-

-

-

9

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

751419

Zhang

Structural insights into the ...

Escherichia coli

J. Struct. Biol.

192

478-486

2015

-

-

1

1

4

-

-

3

-

-

-

1

-

1

-

-

1

-

-

-

-

-

2

-

2

-

-

-

3

-

-

-

-

-

-

-

-

-

1

-

1

4

-

-

-

-

3

-

-

-

1

-

-

-

1

-

-

-

-

2

-

-

-

-

3

-

-

-

-

-

-

-

-

3

3

749471

Hirsch

Transition-state analysis of ...

Homo sapiens

ACS Chem. Biol.

9

2255-2262

2014

-

-

1

-

-

-

-

2

-

-

-

2

-

2

-

-

-

-

-

-

-

-

2

-

2

-

-

-

2

-

-

-

-

-

-

-

-

-

1

-

-

-

-

-

-

-

2

-

-

-

2

-

-

-

-

-

-

-

-

2

-

-

-

-

2

-

-

-

-

-

-

-

-

2

2

751688

Rosenthal

Macrodomain-containing protei ...

Homo sapiens

Nat. Struct. Mol. Biol.

20

502-507

2013

-

-

1

-

1

-

-

-

-

-

-

6

-

5

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1

-

-

-

-

-

13

-

6

-

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-

-

-

3

-

-

1

-

-

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-

6

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3

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-

-

13

-

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-

-

-

1

3

-

-

-

751029

Chen

Identification of macrodomain ...

Escherichia coli, Homo sapiens, Staphylococcus aureus

J. Biol. Chem.

286

13261-13271

2011

-

-

1

1

15

-

3

4

-

-

-

8

-

8

-

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1

-

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1

-

-

12

-

13

-

-

-

18

-

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-

-

1

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1

-

1

15

-

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4

1

4

-

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8

-

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1

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2

-

-

12

-

-

-

-

18

-

-

-

-

-

-

-

-

4

4

751030

Kasamatsu

Hydrolysis of O-acetyl-ADP-ri ...

Homo sapiens

J. Biol. Chem.

286

21110-21117

2011

-

-

-

-

-

-

4

1

-

1

-

1

-

1

-

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-

-

-

-

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-

6

1

3

-

-

-

-

1

-

-

-

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3

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-

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3

4

-

1

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1

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1

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-

-

6

1

-

-

-

-

1

-

-

-

-

-

-

-

-

-

752026

Ono

The 39-kDa poly(ADP-ribose) g ...

Homo sapiens

Proc. Natl. Acad. Sci. USA

103

16687-16691

2006

1

-

-

-

3

-

2

-

-

1

-

2

-

1

-

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-

-

-

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-

2

1

3

-

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-

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-

-

-

-

1

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1

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-

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3

-

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2

1

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1

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2

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-

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-

-

-

-

-

2

1

-

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-

-

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-

-

-

-

-

751012

Rafty

-

Analysis of O-acetyl-ADP-ribo ...

Homo sapiens, Saccharomyces cerevisiae

J. Biol. Chem.

277

47114-47122

2002

-

-

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