Application | Comment | Organism |
---|---|---|
degradation | exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
G62A | exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme. Mutant G62A reveals a 5.5fold lower binding constant to the inhibitor mono-(2-hydroxyethyl) terephthalate than the wild type enzyme | Thermobifida fusca |
G62A/I213S | exchange of amino acid residues of TfCut2 involved in substrate binding with those present in LC-cutinase, UniProt ID G9BY57, from an uncultured bacterium, leads to enzyme variants with increased PET hydrolytic activity at 65°C. Variant causes a weight loss of PET films of more than 42% after 50 h of hydrolysis, corresponding to a 2.7fold increase compared to the wild type enzyme | Thermobifida fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mono-(2-hydroxyethyl) terephthalate | strong inhibition | Thermobifida fusca |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | E5BBQ3 | cf. EC 3.1.1.74 | - |
Thermobifida fusca KW3 | E5BBQ3 | cf. EC 3.1.1.74 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(ethylene terephthalate)n + H2O | substrates are amorphous PET film or PET fibers | Thermobifida fusca | (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate | terephthalate is the dominant hydrolysis product detected after a reaction time of 50 h at 65°C. Monohydroxyethylene terephthalate which inhibits the hydrolysis of PET is almost completely converted into terephthalate and accounts for less than 9.1% of the total hydrolysis products | ? | |
(ethylene terephthalate)n + H2O | substrates are amorphous PET film or PET fibers | Thermobifida fusca KW3 | (ethylene terephthalate)n-1 + 4-[(2-hydroxyethoxy)carbonyl]benzoate | terephthalate is the dominant hydrolysis product detected after a reaction time of 50 h at 65°C. Monohydroxyethylene terephthalate which inhibits the hydrolysis of PET is almost completely converted into terephthalate and accounts for less than 9.1% of the total hydrolysis products | ? |