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Literature summary for 2.8.3.26 extracted from

  • Borjian, F.; Johnsen, U.; Schoenheit, P.; Berg, I.A.
    Succinyl-CoA mesaconate CoA-transferase and mesaconyl-CoA hydratase, enzymes of the methylaspartate cycle in Haloarcula hispanica (2017), Front. Microbiol., 8, 1683 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.3.26

Cloned(Commentary)

Cloned (Comment) Organism
Haloferax volcanii strain H1209 is used for the heterologous expression of the Haloarcula hispanica proteins Haloarcula hispanica

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 5 mM, 5% inhibition Haloarcula hispanica
CoCl2 5 mM, 10% inhibition Haloarcula hispanica
MgCl2 5 mM, 7% inhibition Haloarcula hispanica
MnCl2 5 mM, 24% inhibition Haloarcula hispanica
additional information no inhibition by EDTA Haloarcula hispanica
NiCl2 5 mM, 5% inhibition Haloarcula hispanica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 Methylsuccinate 37°C, pH 7.8, 1 mM succinyl-CoA Haloarcula hispanica
1.3
-
 mesaconate 37°C, pH 7.8, 1 mM succinyl-CoA Haloarcula hispanica
2.8
-
 succinyl-CoA 37°C, pH 7.8 Haloarcula hispanica
2.9
-
 Methylsuccinate 37°C, pH 7.8, 5 mM succinyl-CoA Haloarcula hispanica
7.1
-
 mesaconate 37°C, pH 7.8, 5 mM succinyl-CoA Haloarcula hispanica
39.3
-
 Acrylate 37°C, pH 7.8 Haloarcula hispanica
40.9
-
 Glutarate 37°C, pH 7.8 Haloarcula hispanica

Metals/Ions

Metals/Ions Comment Organism Structure
KCl the enzyme is dependent on the presence of KCl and shows highest activity at 4 M KCl Haloarcula hispanica
additional information no requirement for divalent cations Haloarcula hispanica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 gel filtration Haloarcula hispanica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
mesaconate + succinyl-CoA Haloarcula hispanica the enzyme is involved in the methylaspartate cycle 2-methylfumaryl-CoA + succinate
-
 ?

Organism

Organism UniProt Comment Textmining
Haloarcula hispanica G0HQ31
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Haloarcula hispanica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
16.9
-
 37°C, pH 7.8 Haloarcula hispanica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acrylate + succinyl-CoA low activity Haloarcula hispanica ? + succinate
-
 ?
glutarate + succinyl-CoA low activity Haloarcula hispanica ? + succinate
-
 ?
itaconate + succinyl-CoA low activity Haloarcula hispanica ? + succinate
-
 ?
mesaconate + succinyl-CoA the enzyme is involved in the methylaspartate cycle Haloarcula hispanica 2-methylfumaryl-CoA + succinate
-
 ?
mesaconate + succinyl-CoA whereas the enzyme is highly specific for succinyl-CoA as a CoA-donor, it can activate other carboxylic acids, i.e., methylsuccinate, itaconate, acrylate, and glutarate Haloarcula hispanica 2-methylfumaryl-CoA + succinate
-
 ?
methylsuccinate + succinyl-CoA high activity, not a natural substrate Haloarcula hispanica ? + succinate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 48000, SDS-PAGE Haloarcula hispanica

Synonyms

Synonyms Comment Organism
MCT
-
 Haloarcula hispanica
mesaconate:succinyl-CoA CoA transferase
-
 Haloarcula hispanica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Haloarcula hispanica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
12.2
-
 succinyl-CoA 37°C, pH 7.8 Haloarcula hispanica
12.2
-
 mesaconate 37°C, pH 7.8 Haloarcula hispanica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Haloarcula hispanica

pI Value

Organism Comment pI Value Maximum pI Value
Haloarcula hispanica calculated from sequence
-
 4.3

General Information

General Information Comment Organism
evolution the mct gene is mainly transmitted vertically among haloarchaea Haloarcula hispanica
metabolism the enzyme catalyzes a reaction in the methylaspartate cycle Haloarcula hispanica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.12
-
 Acrylate 37°C, pH 7.8 Haloarcula hispanica
0.32
-
 Glutarate 37°C, pH 7.8 Haloarcula hispanica
4.6
-
 mesaconate 37°C, pH 7.8, 5 mM succinyl-CoA Haloarcula hispanica
9.1
-
 Methylsuccinate 37°C, pH 7.8, 5 mM succinyl-CoA Haloarcula hispanica
9.8
-
 mesaconate 37°C, pH 7.8, 1 mM succinyl-CoA Haloarcula hispanica
12.4
-
 Methylsuccinate 37°C, pH 7.8, 1 mM succinyl-CoA Haloarcula hispanica
17.8
-
 succinyl-CoA 37°C, pH 7.8 Haloarcula hispanica