Cloned (Comment) | Organism |
---|---|
wild-type and mutant genes gctA and gctB encoding the two subunits GctA and GctB are cloned and expressed together in Escherichia coli XL1-Blue | Acidaminococcus fermentans |
Protein Variants | Comment | Organism |
---|---|---|
E54A | mutation in the subunit GctB, inactive mutant | Acidaminococcus fermentans |
E54A | 0.02% as active as wild-type enzyme | Acidaminococcus fermentans |
E54D | mutation in the subunit GctB | Acidaminococcus fermentans |
E54D | above 7% as active as wild-type enzyme, mutant with changed catalytic mechanism without CoASH intermediate, the Escherichia coli strain producing mutant enzyme shows a lower growth rate and reduced amount of recombinant enzyme | Acidaminococcus fermentans |
E54Q | mutation in the subunit GctB | Acidaminococcus fermentans |
E54Q | mutant activity increases from 1% to almost 100% upon incubation with acetyl-CoA and glutaconate at 37°C within 40 h, the substrates induce the conversion of the mutant glutamine residue into the glutamate residue of the wild-type enzyme | Acidaminococcus fermentans |
E64A | mutation in the subunit GctB, 30% as active as wild-type enzyme | Acidaminococcus fermentans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaBH4 | strong | Acidaminococcus fermentans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE | Acidaminococcus fermentans |
36000 | - |
alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE | Acidaminococcus fermentans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (R)-2-hydroxyglutarate | Acidaminococcus fermentans | in the course of glutamate fermentation | acetate + (R)-2-hydroxyglutaryl-CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidaminococcus fermentans | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Acidaminococcus fermentans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + (E)-glutaconate | - |
Acidaminococcus fermentans | acetate + glutaconyl-1-CoA | - |
? | |
acetyl-CoA + (R)-2-hydroxyglutarate | - |
Acidaminococcus fermentans | acetate + (R)-2-hydroxyglutaryl-1-CoA | - |
? | |
acetyl-CoA + (R)-2-hydroxyglutarate | in the course of glutamate fermentation | Acidaminococcus fermentans | acetate + (R)-2-hydroxyglutaryl-CoA | - |
? | |
additional information | residue E-54 of the subunit GctB is directly involved in catalysis by formation of a CoASH ester intermediate | Acidaminococcus fermentans | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha4beta4, 4 * 36000 + 4 * 29000, GctA and GctB, SDS-PAGE | Acidaminococcus fermentans |