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Literature summary for 2.8.2.22 extracted from

  • Kwon, A.R.; Yun, H.J.; Choi, E.C.
    Kinetic mechanism and identification of the active site tyrosine residue in Enterobacter amnigenus arylsulfate sulfotransferase (2001), Biochem. Biophys. Res. Commun., 285, 526-529.
    View publication on PubMed
Search for more literature for 2.8.2.22

Cloned(Commentary)

Cloned (Comment) Organism
-
 Lelliottia amnigena

Protein Variants

Protein Variants Comment Organism
Y101F reduced enzyme activity Lelliottia amnigena
Y123F severely reduced enzyme activity Lelliottia amnigena
Y160F reduced enzyme activity Lelliottia amnigena
Y389F reduced enzyme activity Lelliottia amnigena
Y47F reduced enzyme activity Lelliottia amnigena
Y494F enzyme activity similar to wild-type enzyme Lelliottia amnigena
Y500F reduced enzyme activity Lelliottia amnigena
Y515F reduced enzyme activity Lelliottia amnigena
Y533F reduced enzyme activity Lelliottia amnigena
Y82F reduced enzyme activity Lelliottia amnigena
Y92F increased enzyme activity Lelliottia amnigena

Inhibitors

Inhibitors Comment Organism Structure
1-naphthyl sulfate competitive with 4-nitrophenyl sulfate, noncompetitive with 1-naphthol Lelliottia amnigena
4-nitrophenol noncompetitive with respect to 4-nitrophenyl sulfate, competitive with respect to alpha-naphthol Lelliottia amnigena

Organism

Organism UniProt Comment Textmining
Lelliottia amnigena Q9XDP2 AR-37
-
Lelliottia amnigena AR-37 Q9XDP2 AR-37
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lelliottia amnigena

Reaction

Reaction Comment Organism Reaction ID
an aryl sulfate + a phenol = a phenol + an aryl sulfate mechanism Lelliottia amnigena
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl sulfate + alpha-naphthol
-
 Lelliottia amnigena 4-nitrophenol + 1-naphththyl sulfate
-
 ?
4-nitrophenyl sulfate + alpha-naphthol
-
 Lelliottia amnigena AR-37 4-nitrophenol + 1-naphththyl sulfate
-
 ?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.15
-
 4-nitrophenol pH 9, 37°C, for alpha-naphthol Lelliottia amnigena
0.87
-
 4-nitrophenol pH 9, 37°C, for 4-nitrophenyl sulfate Lelliottia amnigena
1.63
-
 1-naphthyl sulfate pH 9, 37°C, for 1-naphthol Lelliottia amnigena
2.68
-
 1-naphthyl sulfate pH 9, 37°C, for 4-nitrophenyl sulfate Lelliottia amnigena