BRENDA - Enzyme Database show
show all sequences of 2.8.2.22

Molecular cloning of the arylsulfate sulfotransferase gene and characterization of its product from Enterobacter amnigenus AR-37

Kwon, A.R.; Oh, T.G.; Kim, D.H.; Choi, E.C.; Protein Expr. Purif. 17, 366-372 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Lelliottia amnigena
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.163
-
4-nitrophenyl sulfate
pH 9, 37°C, with phenol as second substrate
Lelliottia amnigena
0.314
-
phenol
pH 9, 37°C, with 4-nitrophenyl sulfate as second substrate
Lelliottia amnigena
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63880
-
calculated from nucleotide sequence
Lelliottia amnigena
64000
-
SDS-PAGE, gel filtration, wild-type and mutant enzymes
Lelliottia amnigena
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lelliottia amnigena
Q9XDP2
AR-37
-
Lelliottia amnigena AR-37
Q9XDP2
AR-37
-
Purification (Commentary)
Commentary
Organism
recombinant protein
Lelliottia amnigena
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Lelliottia amnigena
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-naphthyl sulfate + phenol
-
645846
Lelliottia amnigena
2-naphthol + phenyl sulfate
-
-
-
?
4-methylumbelliferyl sulfate + phenol
-
645846
Lelliottia amnigena
4-methylumbelliferol + phenyl sulfate
-
-
-
?
4-nitrophenyl sulfate + 4-acetaminophen
-
645846
Lelliottia amnigena
4-nitrophenol + 4-acetaminophenylsulfate
-
-
-
?
4-nitrophenyl sulfate + 4-acetaminophen
-
645846
Lelliottia amnigena AR-37
4-nitrophenol + 4-acetaminophenylsulfate
-
-
-
?
4-nitrophenyl sulfate + alpha-naphthol
best acceptor
645846
Lelliottia amnigena
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
4-nitrophenyl sulfate + alpha-naphthol
best acceptor
645846
Lelliottia amnigena AR-37
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
4-nitrophenyl sulfate + phenol
-
645846
Lelliottia amnigena
phenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + phenol
-
645846
Lelliottia amnigena AR-37
phenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + resorcinol
-
645846
Lelliottia amnigena
resorcinol O-sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + resorcinol
-
645846
Lelliottia amnigena AR-37
resorcinol O-sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + tyramine
-
645846
Lelliottia amnigena
4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + tyramine
-
645846
Lelliottia amnigena AR-37
4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + tyrosine
-
645846
Lelliottia amnigena
tyrosine O-sulfate + 4-nitrophenol
-
-
-
?
alpha-naphthyl sulfate + phenol
-
645846
Lelliottia amnigena
alpha-naphthol + phenyl sulfate
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 64000, SDS-PAGE
Lelliottia amnigena
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Lelliottia amnigena
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Lelliottia amnigena
calculated from amino acid sequence, isoelectric focussing
-
9
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Lelliottia amnigena
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.163
-
4-nitrophenyl sulfate
pH 9, 37°C, with phenol as second substrate
Lelliottia amnigena
0.314
-
phenol
pH 9, 37°C, with 4-nitrophenyl sulfate as second substrate
Lelliottia amnigena
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
63880
-
calculated from nucleotide sequence
Lelliottia amnigena
64000
-
SDS-PAGE, gel filtration, wild-type and mutant enzymes
Lelliottia amnigena
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant protein
Lelliottia amnigena
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
additional information
-
-
Lelliottia amnigena
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-naphthyl sulfate + phenol
-
645846
Lelliottia amnigena
2-naphthol + phenyl sulfate
-
-
-
?
4-methylumbelliferyl sulfate + phenol
-
645846
Lelliottia amnigena
4-methylumbelliferol + phenyl sulfate
-
-
-
?
4-nitrophenyl sulfate + 4-acetaminophen
-
645846
Lelliottia amnigena
4-nitrophenol + 4-acetaminophenylsulfate
-
-
-
?
4-nitrophenyl sulfate + 4-acetaminophen
-
645846
Lelliottia amnigena AR-37
4-nitrophenol + 4-acetaminophenylsulfate
-
-
-
?
4-nitrophenyl sulfate + alpha-naphthol
best acceptor
645846
Lelliottia amnigena
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
4-nitrophenyl sulfate + alpha-naphthol
best acceptor
645846
Lelliottia amnigena AR-37
4-nitrophenol + 1-napththyl sulfate
-
-
-
?
4-nitrophenyl sulfate + phenol
-
645846
Lelliottia amnigena
phenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + phenol
-
645846
Lelliottia amnigena AR-37
phenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + resorcinol
-
645846
Lelliottia amnigena
resorcinol O-sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + resorcinol
-
645846
Lelliottia amnigena AR-37
resorcinol O-sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + tyramine
-
645846
Lelliottia amnigena
4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + tyramine
-
645846
Lelliottia amnigena AR-37
4-(2-aminoethyl)phenyl sulfate + 4-nitrophenol
-
-
-
?
4-nitrophenyl sulfate + tyrosine
-
645846
Lelliottia amnigena
tyrosine O-sulfate + 4-nitrophenol
-
-
-
?
alpha-naphthyl sulfate + phenol
-
645846
Lelliottia amnigena
alpha-naphthol + phenyl sulfate
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 64000, SDS-PAGE
Lelliottia amnigena
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Lelliottia amnigena
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Lelliottia amnigena
calculated from amino acid sequence, isoelectric focussing
-
9
Other publictions for EC 2.8.2.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737843
Roubalova
Sulfation modulates the cell u ...
Desulfitobacterium hafniense
Bioorg. Med. Chem.
23
5402-5409
2015
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-
-
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-
-
-
-
-
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4
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3
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3
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-
739142
Tang
A two-step sulfation in antibi ...
Streptomyces sp., Streptomyces sp. MK730-62F2
Nat. Chem. Biol.
9
610-615
2013
-
-
-
-
-
-
-
-
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-
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3
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2
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2
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-
-
723897
Van Der Horst
-
Sulfation of various alcoholic ...
Desulfitobacterium hafniense
Adv. Synth. Catal.
354
3501-3508
2012
-
-
1
-
-
-
4
-
-
-
2
1
1
1
-
-
1
-
-
-
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-
33
1
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1
-
1
1
-
-
-
-
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-
1
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-
4
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2
1
1
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1
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-
33
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
725393
Kaysser
A new arylsulfate sulfotransfe ...
Streptomyces sp., Streptomyces sp. MK730-62F2
J. Biol. Chem.
285
12684-12694
2010
1
-
1
-
3
-
1
4
-
1
1
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-
5
-
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1
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-
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8
1
-
-
-
2
1
1
-
-
2
-
-
1
-
1
-
-
3
-
-
1
2
4
-
1
1
-
-
-
-
1
-
-
-
-
8
1
-
-
-
2
1
1
-
-
-
-
-
-
-
-
693650
Grimshaw
DsbL and DsbI form a specific ...
Escherichia coli
J. Mol. Biol.
380
667-680
2008
1
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1
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1
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1
1
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2
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1
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2
1
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1
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2
1
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-
-
-
-
-
706490
Malojcic
A structural and biochemical b ...
Escherichia coli, Escherichia coli CFT073
Proc. Natl. Acad. Sci. USA
105
19217-19222
2008
-
-
-
-
-
-
1
2
1
-
2
2
-
6
-
-
-
-
-
-
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-
8
1
-
-
-
1
-
-
-
1
2
-
-
-
-
-
1
-
-
-
-
1
2
2
1
-
2
2
-
-
-
-
-
-
-
-
8
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
672493
Kim
Cloning, expression and purifi ...
Eubacterium sp., Eubacterium sp. A-44
Biol. Pharm. Bull.
30
11-14
2007
-
-
1
-
-
-
-
-
-
-
1
-
-
10
-
-
1
-
-
-
2
-
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
-
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-
1
-
-
-
-
1
-
-
2
-
8
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
660838
Kwon
Role of disulfide bond of aryl ...
Lelliottia amnigena
Arch. Pharm. Res.
28
561-565
2005
1
-
1
-
7
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645849
Mozhaev
Arylsulfotransferase from Clos ...
Clostridium innocuum, Clostridium innocuum 554
Biotechnol. Bioeng.
78
567-575
2002
-
-
-
-
-
-
-
-
-
-
2
-
10
2
-
-
1
-
-
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-
-
4
1
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
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-
-
-
-
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-
2
-
10
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645844
Kang
Cloning, sequence analysis, an ...
Citrobacter freundii
Arch. Pharm. Res.
24
316-322
2001
-
-
1
-
-
-
-
-
-
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-
-
7
-
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-
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6
-
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1
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6
-
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-
-
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-
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-
-
-
-
645847
Kwon
Kinetic mechanism and identifi ...
Lelliottia amnigena, Lelliottia amnigena AR-37
Biochem. Biophys. Res. Commun.
285
526-529
2001
-
-
1
-
11
-
2
-
-
-
-
-
-
4
-
-
1
1
-
-
-
-
2
-
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-
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4
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1
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11
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2
4
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1
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2
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645843
Goldberg
Purification, cloning, and cha ...
Eubacterium rectale, Eubacterium rectale IIIH
J. Ind. Microbiol. Biotechnol.
25
305-309
2000
-
-
1
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1
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1
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1
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1
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1
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1
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1
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-
645846
Kwon
Molecular cloning of the aryls ...
Lelliottia amnigena, Lelliottia amnigena AR-37
Protein Expr. Purif.
17
366-372
1999
-
-
1
-
-
-
-
2
-
-
2
-
-
8
-
-
1
-
-
-
1
-
14
1
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-
-
-
1
-
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-
-
1
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-
-
1
-
-
-
-
-
-
-
2
-
-
2
-
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-
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1
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-
1
-
14
1
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-
-
-
1
-
-
1
-
-
-
-
-
-
645841
Bidwell
Bacterial expression of two hu ...
Homo sapiens
Chem. Biol. Interact.
109
137-141
1998
-
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1
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1
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1
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1
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1
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1
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1
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645845
Konishi-Imamura
Regulation of arylsulfate sulf ...
Eubacterium sp.
J. Enzyme Inhib.
8
233-241
1995
-
-
-
-
-
-
6
-
-
2
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-
-
3
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
6
4
-
-
2
-
-
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645848
Lee
Purification and reaction mech ...
Haemophilus sp., Haemophilus sp. K-12
J. Biochem.
118
796-801
1995
-
-
-
-
-
-
7
2
-
-
2
-
-
5
-
-
1
1
-
3
1
-
11
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
7
4
2
-
-
2
-
-
-
-
1
-
3
1
-
11
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645839
Konishi-Imamura
Improved purification of aryls ...
Eubacterium sp., Eubacterium sp. A-44
J. Biochem.
115
1097-1100
1994
-
-
-
-
-
-
-
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2
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11
-
-
1
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2
1
-
4
-
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-
-
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-
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-
-
2
-
-
-
1
-
2
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
645842
Muramatsu
Enzymic O-sulfation of tyrosin ...
Eubacterium sp., Eubacterium sp. A-44
Eur. J. Biochem.
223
243-248
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
645840
Kim
Purification and characterizat ...
Klebsiella sp., Klebsiella sp. K-36
J. Biochem.
112
456-460
1992
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-
-
-
-
-
7
2
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2
2
2
-
2
-
-
1
-
-
-
1
-
14
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
2
-
2
2
2
-
-
-
1
-
-
1
-
14
1
1
-
2
-
1
-
-
-
-
-
-
-
-
-
645837
Kobashi
-
A novel type of arylsulfotrans ...
Eubacterium sp., Eubacterium sp. A-44
J. Protein Chem.
6
237-244
1987
2
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-
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-
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1
2
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-
7
-
-
1
1
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-
-
-
14
1
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
-
-
14
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
645838
Kim
Purification, characterization ...
Eubacterium sp., Eubacterium sp. A-44
Biochim. Biophys. Acta
872
33-41
1986
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-
-
-
-
1
11
2
-
2
2
-
-
7
-
-
1
1
-
-
1
1
8
1
1
-
2
-
3
-
1
-
-
1
-
-
-
-
-
-
-
1
-
11
-
2
-
2
2
-
-
-
-
1
-
-
1
1
8
1
1
-
2
-
3
-
1
1
-
-
-
-
-
-
645850
Kobashi
A novel sulfotransferase sulfa ...
Eubacterium sp., Eubacterium sp. A-44
Biochem. Biophys. Res. Commun.
140
38-42
1986
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7
-
-
-
-
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-
1
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12
-
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-
1
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-
-
-
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-
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-
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-
-
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-
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-
-
1
-
12
-
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-
1
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-