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Literature summary for 2.8.2.20 extracted from

  • Lin, W.; Larsen, K.; Hortin, G.L.; Roth, J.A.
    Recognition of substrates by tyrosylprotein sulfotransferase. Determination of affinity by acidic amino acids near the target sites (1992), J. Biol. Chem., 267, 2876-2879.
    View publication on PubMed
Search for more literature for 2.8.2.20

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by Glu, Asp, Gln, Asn, Ser Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic properties with synthetic peptides as substrates, with modified lengths on NH2- or COOH-termini, changes in Km-values resulting from substitution of negatively charged amino acids in the region of the acceptor tyrosine Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi membrane
-
 Rattus norvegicus 139
-
membrane
-
 Rattus norvegicus 16020
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
liver Rattus norvegicus
partial Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate mechanism Rattus norvegicus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-80°C, in 10 mM HEPES-buffer, pH 7, 50 mM NaCl, 1% Triton X-100, 1 mM DTT, 25% glycerol, several months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + synthetic peptides highly site-specific Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?
3'-phosphoadenylylsulfate + synthetic peptides structure based on sequences surrounding the sulfated Tyr of naturally sulfated proteins Rattus norvegicus adenosine 3',5'-bisphosphate + ?
-
 ?