Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.8.2.2 extracted from

  • Feng, L.; Yuen, Y.L.; Xu, J.; Liu, X.; Chan, M.Y.; Wang, K.; Fong, W.P.; Cheung, W.T.; Lee, S.S.
    Identification and characterization of a novel PPARalpha-regulated and 7alpha-hydroxyl bile acid-preferring cytosolic sulfotransferase mL-STL (Sult2a8) (2017), J. Lipid Res., 58, 1114-1131 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.2

Cloned(Commentary)

Cloned (Comment) Organism
gene SULT2B1b, quantitative RT-PCR enzyme expression analysis, recombinant overexpression in LNCaP and DU-145 prostate cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of SULT2B1b knockdown prostate cells using siRNA or shRNA targeting and LNCaP cells. SULT2B1b knockdown results in a decrease in growth/viability of LNCaP, VCaP, C4-2, and RWPE-1 cells as well as decreased softagar colony formation in LNCaP cells, and it induces cell death in prostate cancer cells. A significant decrease in cell growth/viability is observed using multiple SULT2B1b RNAi sequences in LNCaP cells. SULT2B1b knockdown increases the percentage of sub-G1 nuclei by cell-cycle analysis and significantly increased caspase-3 activity and PARP cleavage in LNCaP, VCaP, and C4-2 cells. LNCaP cells with SULT2B1b knockdown analyzed by flow cytometry show an increased percentage of Annexin V+/propidium iodide (PI)x02cells compared with control cells. Double KD of SULT2B1b/LXRbeta does not affect the siRNA knockdown efficiency compared with SULT2B1b or LXRbeta knockdown alone. LNCaP cells with SULT2B1b overexpression (hSULT2B1b vector) show a significant decrease in liver X receptor (LXR) activity and a trend of decreased transcription of downstream target gene, ATP-binding cassette (ABC)-G1. LXR activity in LNCaP cells has been demonstrated to be due to transcriptional activation of LXRbeta. Phenotype of SULT2B1b knockout cells, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylyl sulfate + cholesterol Homo sapiens
-
 adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens O00204
-
-

Source Tissue

Source Tissue Comment Organism Textmining
DU-145 cell
-
 Homo sapiens
-
LNCaP cell
-
 Homo sapiens
-
LNCaP-C4-2 cell
-
 Homo sapiens
-
additional information androgen responsive cell lines LNCaP, VCaP, RWPE-1, and the castration nonresponsive line C4-2 generally express higher levels of SULT2B1b than AR- cell lines, such as PC-3 and DU-145 cells Homo sapiens
-
PC-3 cell
-
 Homo sapiens
-
prostate gland
-
 Homo sapiens
-
prostate gland cancer cell
-
 Homo sapiens
-
RWPE-1 cell
-
 Homo sapiens
-
VCaP cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + cholesterol
-
 Homo sapiens adenosine 3',5'-bisphosphate + cholesterol 3-sulfate
-
 ?

Synonyms

Synonyms Comment Organism
cholesterol sulfotransferase
-
 Homo sapiens
SULT2B1b
-
 Homo sapiens

General Information

General Information Comment Organism
malfunction SULT2B1b knockdown induces cell death in prostate cancer cells. SULT2B1b knockdown increases the percentage of sub-G1 nuclei by cell-cycle analysis and significantly increased caspase-3 activity and PARP cleavage in LNCaP, VCaP, and C4-2 cells. Targeted knockdown of SULT2B1b impairs growth/viability of prostate cancer cells and induces apoptosis. LNCaP cells with SULT2B1b overexpression (hSULT2B1b vector) show a significant decrease in liver X receptor (LXR) activity and a trend of decreased transcription of downstream target gene, ATP-binding cassette (ABC)-G1. LXR activity in LNCaP cells has been demonstrated to be due to transcriptional activation of LXRbeta Homo sapiens
physiological function role of SULT2B1b in the growth and progression of cancer cells. Cholesterol sulfate accumulation correlates with SULT2B1b expression in prostate cancer cells and human prostate specimens. SULT2B1b activity modulates androgen receptor (AR) activity in prostate cancer cells, SULT2B1b is a regulator of AR activity and cell growth in prostate cancer Homo sapiens