Cloned (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, comparison of sequence and genetic structure, overview, overexpression of GST-tagged isozymes in Escherichia coli strain BL21(DE3) | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis of recombinant isozymes SULT2B1a and SULT2B1b | Rattus norvegicus | |
0.0017 | - |
cholesterol | pH 7.5, 37°C, recombinant isozyme SULT2B1b | Rattus norvegicus | |
0.0089 | - |
pregnenolone | pH 7.5, 37°C, recombinant isozyme SULT2B1a | Rattus norvegicus | |
0.0179 | - |
dehydroepiandrosterone | pH 7.5, 37°C, recombinant isozyme SULT2B1a | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38319 | - |
x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation | Rattus norvegicus |
42178 | - |
x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + cholesterol | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + dehydroepiandrosterone | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + pregnenolone | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q29YR5 | isoform SULT2B1a | - |
Rattus norvegicus | Q29YR5 | isoform SULT2B1b | - |
Rattus norvegicus | Q29YR5 | SULT2B1; Sprague-Dawley rats, gene SULT2B1, isozymes SULT2B1a and SULT2B1b as a result of an alternative exon I | - |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
recombinant GST-tagged isozymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | isoform SULT2B1a | Rattus norvegicus | - |
brain | SULT2B1a is only expressed in brain and testis | Rattus norvegicus | - |
intestine | isoform SULT2B1b | Rattus norvegicus | - |
intestine | main expression of SULT2B1b | Rattus norvegicus | - |
kidney | isoform SULT2B1b | Rattus norvegicus | - |
kidney | main expression of SULT2B1b | Rattus norvegicus | - |
additional information | tissue distribution of isozymes SULT2B1a and SULT2B1b expression, overview | Rattus norvegicus | - |
skin | isoform SULT2B1b | Rattus norvegicus | - |
skin | main expression of SULT2B1b | Rattus norvegicus | - |
testis | isoform SULT2B1a | Rattus norvegicus | - |
testis | SULT2B1a is only expressed in brain and testis | Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + cholesterol | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + cholesteryl 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + dehydroepiandrosterone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + dehydroepiandrosterone 3-sulfate | - |
? | |
3'-phosphoadenylyl sulfate + pregnenolone | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + pregnenolone 3-sulfate | - |
? | |
3'-phosphoadenylylsulfate + cholesterol | activity is higher than with pregnenolone, isoform SULT2B1b | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + cholesterol | poor activity, isoform SULT2B1a | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + pregnenolone | isoform SULT2B1a | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylylsulfate + pregnenolone | isoform SULT2B1b | Rattus norvegicus | adenosine 3',5'-bisphosphate + ? | - |
? | |
additional information | no activity with dehydroepiandrosterone, isoform SULT2b1a | Rattus norvegicus | ? | - |
? | |
additional information | no activity with dehydroepiandrosterone, isoform SULT2b1b | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 42178, SULT2B1a, sequence calculation, x * 38319, SULT2B1b, sequence calculation | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
steroid/sterol sulfotransferase | - |
Rattus norvegicus |
SULT2B1 | - |
Rattus norvegicus |
SULT2B1a | - |
Rattus norvegicus |
SULT2B1b | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | sequence calculation, SULT2B1b | - |
5.12 |
Rattus norvegicus | sequence calculation, SULT2B1a | - |
5.93 |