Application | Comment | Organism |
---|---|---|
synthesis | the enzyme is used for large scale chondroitin sulfate synthesis in a coupled system with aryl sulfotransferase IV and chondroitin 4-sulfotransferase, overview | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
gene C6ST, recombinant expression in Bacillus subtilis strain E168C, Escherichia coli strain BL21(DE3), and Pichia pastoris strain GS115, coexpression with ASSTIV and C4ST from Rattus norvegicus, subcloning in Escherichia coli strain JM109. Chondroitin extraction and quantification | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | reconstitution of the chondroitin biosynthesis pathway in a recombinant Bacillus subtilis strain using sucrose as carbon source, method optimization, overview. Specific sulfation transformation systems are constructed and optimized by combining the purified aryl sulfotransferase IV (ASST IV, EC 2.8.2.1), chondroitin 4-sulfotransferase (C4ST, EC 2.8.2.5) and chondroitin 6-sulfotransferase (C6ST). Chondroitin sulfate A and C are enzymatically transformed from chondroitin at conversion rates of 98% and 96%, respectively. The ASST IV enzyme is used for PAPS regeneration. Purification of CS disaccharides by anion exchange chrmatography and NMR analysis of the composition of CS disaccharides | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + chondroitin | Rattus norvegicus | - |
adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q9QZL2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + chondroitin | - |
Rattus norvegicus | adenosine 3',5'-bisphosphate + chondroitin 6'-sulfate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C6ST | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rattus norvegicus |