Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Haliangium ochraceum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
3'-phosphoadenylyl sulfate | at pH 7.0 and 25°C | Haliangium ochraceum | |
0.0459 | - |
4-nitrophenyl sulfate | at pH 7.0 and 25°C | Haliangium ochraceum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
x * 40000, SDS-PAGE | Haliangium ochraceum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haliangium ochraceum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-IDA agarose column chromatography | Haliangium ochraceum |
Storage Stability | Organism |
---|---|
4°C, the enzyme retains over 90% of its activity for several days | Haliangium ochraceum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3'-phosphoadenylyl sulfate + 4,4'-biphenol | best substrate with 93% activity | Haliangium ochraceum | adenosine 3',5'-bisphosphate + ? | - |
? | |
3'-phosphoadenylyl sulfate + 4-nitrophenyl sulfate | - |
Haliangium ochraceum | 4-nitrophenol + adenosine 3',5'-bisphosphate | - |
? | |
3'-phosphoadenylyl sulfate + catechol | - |
Haliangium ochraceum | adenosine 3',5'-bisphosphate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Haliangium ochraceum |
? | x * 37221, calculated from amino acid sequence | Haliangium ochraceum |
Synonyms | Comment | Organism |
---|---|---|
ArylST | - |
Haliangium ochraceum |
AST | - |
Haliangium ochraceum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | there is a significant decrease of the half-life of the enzyme between 25 and 37°C. At 25°C the half-life is over 3 h, while at 37°C it is 36-37 min, and at 45°C less than 6 min | Haliangium ochraceum |