Literature summary for 2.8.2.1 extracted from

Dajani, R.; Sharp, S.; Graham, S.; Bethell, S.S.; Cooke, R.M.; Jamieson, D.J.; Coughtrie, M.W.
Kinetic properties of human dopamine sulfotransferase (SULT1A3) expressed in prokaryotic and eukaryotic systems: comparison with the recombinant enzyme purified from Escherichia coli (1999), Protein Expr. Purif., 16, 11-18.

Search for more literature for 2.8.2.1

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis, expression of SULT1A3 in several expression systems: Escherichia coli, Saccharomyces cerevisiae, COS-7 cells, V79 cells, overview	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E146A	-	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.00065	-	dopamine	wild-type SULT1A3: purified recombinant enzyme from Escherichia coli, pH 7.4, 37°C	Homo sapiens
0.00094	-	dopamine	wild-type SULT1A3: recombinant from V79 cells, pH 7.4, 37°C	Homo sapiens
0.001	-	dopamine	wild-type SULT1A3: native enzyme, and recombinant from COS-7 cells, Escherichia coli, Saccharomyces cerevisiae, pH 7.4, 37°C	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P0DMM9	SULT1A3, is a M-PST enzyme form	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant SULT1A3 from Escherichia coli, to homogeneity	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.2	-	purified recombinant SULT1A3	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3'-phosphoadenylyl sulfate + a phenol	-	Homo sapiens	adenosine 3',5'-bisphosphate + an aryl sulfate	-	?

Synonyms

Synonyms	Comment	Organism
SULT	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)