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Literature summary for 2.8.1.B3 extracted from

  • Rajakovich, L.J.; Tomlinson, J.; Dos Santos, P.C.
    Functional analysis of Bacillus subtilis genes involved in the biosynthesis of 4-thiouridine in tRNA (2012), J. Bacteriol., 194, 4933-4940.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.B3

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Arctic Express cells Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bacillus subtilis the enzyme is not able to enhance cysteine desulfurase NifZ activity of sulfide or alanine formation ?
-
 ?
additional information Bacillus subtilis PS832 the enzyme is not able to enhance cysteine desulfurase NifZ activity of sulfide or alanine formation ?
-
 ?
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) Bacillus subtilis
-
 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8) Bacillus subtilis PS832
-
 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis PS832
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, Ni-NTA column chromatography and Sephacryl S-200 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is not able to enhance cysteine desulfurase NifZ activity of sulfide or alanine formation Bacillus subtilis ?
-
 ?
additional information the enzyme is not able to enhance cysteine desulfurase NifZ activity of sulfide or alanine formation Bacillus subtilis PS832 ?
-
 ?
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)
-
 Bacillus subtilis sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?
sulfurtransferase IscS-SSH + adenylated-tRNA-uridine(position8)
-
 Bacillus subtilis PS832 sulfurtransferase IscS-SH + tRNA-4-thiouridine(position8) + AMP
-
 ?

Subunits

Subunits Comment Organism
? x 45400, SDS-PAGE Bacillus subtilis

Synonyms

Synonyms Comment Organism
ThiI
-
 Bacillus subtilis

General Information

General Information Comment Organism
malfunction Bacillus subtilis strains lacking thiI display a specific disruption in the C-4 thiolation of the uridine base at position 8 of tRNA synthesis Bacillus subtilis
metabolism the enzyme is crucial for the formation of C-4 thiolation of the uridine base at position 8 of tRNA modification in tRNA Bacillus subtilis