Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DL41 cells | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
C101/C430A | inactive | Arabidopsis thaliana |
C151/C430A | inactive | Arabidopsis thaliana |
C206S | the mutants activity is reduced to 18% | Arabidopsis thaliana |
C206S/C430A | inactive | Arabidopsis thaliana |
C250/C430A | inactive | Arabidopsis thaliana |
C377/C430A | inactive | Arabidopsis thaliana |
C428/435A | the mutant shows wild type sensitivity towards N-ethylmaleimide | Arabidopsis thaliana |
C435A | the mutant is less sensitive towards N-ethylmaleimide compared to the wild type enzyme | Arabidopsis thaliana |
C456/C430A | inactive | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | less than 80% activity at 1 mM, 30% activity at 1.5 mM, complete inhibition at 2 mM | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
molybdenum cofactor + L-cysteine + H+ | Arabidopsis thaliana | the enzyme consists of an N-terminal NifS-like domain that exhibits L-cysteine desulfurase activity and a C-terminal domain that binds sulfurated molybdenum cofactor | thio-molybdenum cofactor + L-alanine + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9C5X8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
molybdenum cofactor + L-cysteine + H+ | the enzyme consists of an N-terminal NifS-like domain that exhibits L-cysteine desulfurase activity and a C-terminal domain that binds sulfurated molybdenum cofactor | Arabidopsis thaliana | thio-molybdenum cofactor + L-alanine + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ABA3 | - |
Arabidopsis thaliana |
cysteine desulfurase | - |
Arabidopsis thaliana |
molybdenum cofactor sulfurase | - |
Arabidopsis thaliana |