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Literature summary for 2.8.1.7 extracted from

  • Dunkle, J.; Bruno, M.; Frantom, P.
    Structural evidence for a latch mechanism regulating access to the active site of SufS-family cysteine desulfurases (2020), Acta Crystallogr. Sect. D, 76, 291-301 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.7

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of SufS including a structure containing an inward-facing persulfide intermediate on residue C364. Structures of SufS variants with substitutions at the dimer interface show changes in dimer geometry, a conserved beta-hairpin structure may play a role in mediating interactions with SufE Escherichia coli

Protein Variants

Protein Variants Comment Organism
E250A about 7fold decrease in kcat/Km value, mutant displays altered dimer geometries Escherichia coli
E96A about 6fold decrease in kcat/Km value, mutant displays altered dimer geometries Escherichia coli
H55A about 3fold increase in kcat/Km value Escherichia coli
R92A about 3fold decrease in kcat/Km value, dimer geometry is identical to that of wild-type Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 L-cysteine wild-type, pH 8.0, temperature not specified in the publication Escherichia coli
0.075
-
 L-cysteine mutant H55A, pH 8.0, temperature not specified in the publication Escherichia coli
0.081
-
 L-cysteine mutant E250A, pH 8.0, temperature not specified in the publication Escherichia coli
0.095
-
 L-cysteine mutant E96A, pH 8.0, temperature not specified in the publication Escherichia coli
0.095
-
 L-cysteine mutant R92A, pH 8.0, temperature not specified in the publication Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P77444
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + acceptor overall reaction Escherichia coli L-alanine + S-sulfanyl-acceptor
-
 ?

Synonyms

Synonyms Comment Organism
SufS
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.123
-
 L-cysteine mutant R92A, pH 8.0, temperature not specified in the publication Escherichia coli
0.167
-
 L-cysteine mutant E250A, pH 8.0, temperature not specified in the publication Escherichia coli
0.202
-
 L-cysteine wild-type, pH 8.0, temperature not specified in the publication Escherichia coli
0.23
-
 L-cysteine mutant E96A, pH 8.0, temperature not specified in the publication Escherichia coli
0.65
-
 L-cysteine mutant H55A, pH 8.0, temperature not specified in the publication Escherichia coli

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2
-
 L-cysteine mutant E250A, pH 8.0, temperature not specified in the publication Escherichia coli
1.3
-
 L-cysteine mutant R92A, pH 8.0, temperature not specified in the publication Escherichia coli
2.3
-
 L-cysteine mutant E96A, pH 8.0, temperature not specified in the publication Escherichia coli
4
-
 L-cysteine wild-type, pH 8.0, temperature not specified in the publication Escherichia coli
8.3
-
 L-cysteine mutant H55A, pH 8.0, temperature not specified in the publication Escherichia coli