Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
mitochondrion | predominantly localized in the mitochondria | Homo sapiens | 5739 | - |
nucleus | - |
Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
2 * 45000, SDS-PAGE | Homo sapiens |
50000 | - |
x * 50000, SDS-PAGE | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + MOC3 protein | Homo sapiens | - |
L-alanine + S-sulfanyl-MOC3 protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + MOC3 protein | - |
Homo sapiens | L-alanine + S-sulfanyl-MOC3 protein | - |
? | |
L-cysteine + [enzyme]-cysteine | - |
Homo sapiens | L-alanine + [enzyme]-S-sulfanylcysteine | - |
? | |
additional information | NFS1 acts as the sulfur donor for the C-terminal domain of MOCS3 protein (a cytosolic protein involved in molybdenum cofactor biosynthesis and tRNA thiolation) | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 50000, SDS-PAGE | Homo sapiens |
homodimer | 2 * 45000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
L-cysteine desulfurase | - |
Homo sapiens |
Nfs1 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is the sulfur donor for M molybdenum cofactor biosynthesis. The enzyme is able to transfer sulfur to the C-terminal domain of MOCS3, a cytosolic protein involved in molybdenum cofactor biosynthesis and tRNA thiolation | Homo sapiens |
physiological function | the enzyme provides the sulfur for molybdenum cofactor biosynthesis in human cytosol. The enzyme plays a crucial role in the mitochondrial iron-sulfur cluster biosynthesis and in the thiomodification of mitochondrial and cytosolic tRNAs | Homo sapiens |