BRENDA - Enzyme Database
show all sequences of 2.8.1.3

Purification and steady-state kinetic analysis of yeast thiosulfate reductase

Uhteg, L.; Westley, J.; Arch. Biochem. Biophys. 195, 211-222 (1979)

Data extracted from this reference:

General Stability
General Stability
Organism
freezing and thawing during the later stages of purification destabilize
Saccharomyces cerevisiae
Inhibitors
Inhibitors
Commentary
Organism
Structure
benzene thiosulfonate
competitive with both substrates
Saccharomyces cerevisiae
oxidized glutathione
product inhibition, competitive with glutathione
Saccharomyces cerevisiae
sulfate
-
Saccharomyces cerevisiae
Sulfide
product inhibition, competitive versus both substrates
Saccharomyces cerevisiae
sulfite
product inhibition, competitive versus both substrates
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
SDS-PAGE, gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
thiosulfate + glutathione
Saccharomyces cerevisiae
-
sulfite + glutathione disulfide + sulfide
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
Red Star brand
-
Purification (Commentary)
Commentary
Organism
70fold
Saccharomyces cerevisiae
Reaction
Reaction
Commentary
Organism
thiosulfate + 2 glutathione = sulfite + glutathione disulfide + sulfide
mechanism, substrates add in a random fashion
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
62
-
-
Saccharomyces cerevisiae
Storage Stability
Storage Stability
Organism
-35°C, concentrated enzyme in solution of 50 mM Tris, pH 8.0, 0.5 mM NaS2O3, 50% glycerol, stable for at least 4 months
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-cysteine + glutathione
-
645570
Saccharomyces cerevisiae
?
-
-
-
?
additional information
the enzyme has two distinct closely situated substrate binding sites, one for compounds with an RSO3-structure and one for the sulfhydryl substrate
645570
Saccharomyces cerevisiae
?
-
-
-
-
thiosulfate + glutathione
-
645570
Saccharomyces cerevisiae
sulfite + glutathione disulfide + sulfide
-
-
-
?
thiosulfate + glutathione
-
645570
Saccharomyces cerevisiae
sulfite + glutathione disulfide + sulfide
the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide
645570
Saccharomyces cerevisiae
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 17000, SDS-PAGE
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Saccharomyces cerevisiae
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Saccharomyces cerevisiae
isoelectric focusing using ampholytes pH 4-6 and pH 2-10
-
5.1
General Stability (protein specific)
General Stability
Organism
freezing and thawing during the later stages of purification destabilize
Saccharomyces cerevisiae
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
benzene thiosulfonate
competitive with both substrates
Saccharomyces cerevisiae
oxidized glutathione
product inhibition, competitive with glutathione
Saccharomyces cerevisiae
sulfate
-
Saccharomyces cerevisiae
Sulfide
product inhibition, competitive versus both substrates
Saccharomyces cerevisiae
sulfite
product inhibition, competitive versus both substrates
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
17000
-
SDS-PAGE, gel filtration
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
thiosulfate + glutathione
Saccharomyces cerevisiae
-
sulfite + glutathione disulfide + sulfide
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
70fold
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
62
-
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability
Organism
-35°C, concentrated enzyme in solution of 50 mM Tris, pH 8.0, 0.5 mM NaS2O3, 50% glycerol, stable for at least 4 months
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-cysteine + glutathione
-
645570
Saccharomyces cerevisiae
?
-
-
-
?
additional information
the enzyme has two distinct closely situated substrate binding sites, one for compounds with an RSO3-structure and one for the sulfhydryl substrate
645570
Saccharomyces cerevisiae
?
-
-
-
-
thiosulfate + glutathione
-
645570
Saccharomyces cerevisiae
sulfite + glutathione disulfide + sulfide
-
-
-
?
thiosulfate + glutathione
-
645570
Saccharomyces cerevisiae
sulfite + glutathione disulfide + sulfide
the primary product is glutathione hydrodisulfide, which reacts with glutathione to give oxidized glutathione and sulfide
645570
Saccharomyces cerevisiae
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 17000, SDS-PAGE
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9
-
-
Saccharomyces cerevisiae
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Saccharomyces cerevisiae
isoelectric focusing using ampholytes pH 4-6 and pH 2-10
-
5.1
Other publictions for EC 2.8.1.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
645574
Aird
A physical characterization of ...
Acinetobacter calcoaceticus
Biochim. Biophys. Acta
1038
10-17
1990
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2
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1
-
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-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
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-
-
1
-
-
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-
-
-
-
-
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-
-
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-
-
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-
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-
645519
Aird
Isolation and characterization ...
Acinetobacter calcoaceticus
J. Biol. Chem.
262
17327-17335
1987
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2
2
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-
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-
3
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-
1
1
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-
1
-
2
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
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1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
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645571
Chauncey
Improved purification and sulf ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
744
304-311
1983
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1
1
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-
1
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1
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1
1
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1
1
2
-
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-
1
-
1
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1
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1
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1
1
2
-
-
-
-
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-
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-
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645572
Chauncey
The catalytic mechanism of yea ...
Saccharomyces cerevisiae
J. Biol. Chem.
258
15037-15045
1983
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3
4
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-
1
-
1
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-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
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-
-
-
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-
-
-
-
-
-
-
3
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4
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
645570
Uhteg
Purification and steady-state ...
Saccharomyces cerevisiae
Arch. Biochem. Biophys.
195
211-222
1979
-
-
-
-
-
1
5
-
-
-
1
1
-
1
-
-
1
1
-
-
1
1
4
1
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
5
-
-
-
-
1
1
-
-
-
1
-
-
1
1
4
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-