BRENDA - Enzyme Database
show all sequences of 2.8.1.10

Structural Basis for iron-mediated sulfur transfer in archael and yeast thiazole synthases

Zhang, X.; Eser, B.E.; Chanani, P.K.; Begley, T.P.; Ealick, S.E.; Biochemistry 55, 1826-1838 (2016) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expressed in Escherichia coli BL21(DE3) cells
Methanocaldococcus jannaschii
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
expressed in Escherichia coli BL21(DE3) cells
Methanotorris igneus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
C205S variant with a bound glycine imine intermediate, hanging drop vapor diffusion method, using 25% (w/v) PEG1500, 0.0125 M succinic acid, 0.044 M sodium dihydrogen phosphate, and 0.044 M glycine, pH 8.5
Saccharomyces cerevisiae
wild type enzyme complexed with ADP-ribulose, hanging drop vapor diffusion method, using 1.05 M potassium/sodium tartrate, 0.1 M CHES/sodium hydroxide, pH 9.5 and 0.2 M lithium sulfate
Methanocaldococcus jannaschii
wild type enzyme with bound glycine imine intermediate and iron, hanging drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M CHES/sodium hydroxide, pH 9.5
Methanotorris igneus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Methanocaldococcus jannaschii
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Saccharomyces cerevisiae
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Methanotorris igneus
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Methanocaldococcus jannaschii
-
-
-
Methanotorris igneus
-
-
-
Saccharomyces cerevisiae
P32318
-
-
Purification (Commentary)
Purification (Commentary)
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Methanocaldococcus jannaschii
Ni-NTA column chromatography and Superdex 200 gel filtration
Saccharomyces cerevisiae
Ni-NTA column chromatography and Superdex 200 gel filtration
Methanotorris igneus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Methanocaldococcus jannaschii
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Saccharomyces cerevisiae
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Methanotorris igneus
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Thi4
-
Methanocaldococcus jannaschii
Thi4
-
Saccharomyces cerevisiae
Thi4
-
Methanotorris igneus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Methanocaldococcus jannaschii
expressed in Escherichia coli BL21(DE3) cells
Saccharomyces cerevisiae
expressed in Escherichia coli BL21(DE3) cells
Methanotorris igneus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
C205S variant with a bound glycine imine intermediate, hanging drop vapor diffusion method, using 25% (w/v) PEG1500, 0.0125 M succinic acid, 0.044 M sodium dihydrogen phosphate, and 0.044 M glycine, pH 8.5
Saccharomyces cerevisiae
wild type enzyme complexed with ADP-ribulose, hanging drop vapor diffusion method, using 1.05 M potassium/sodium tartrate, 0.1 M CHES/sodium hydroxide, pH 9.5 and 0.2 M lithium sulfate
Methanocaldococcus jannaschii
wild type enzyme with bound glycine imine intermediate and iron, hanging drop vapor diffusion method, using 1.0 M sodium citrate, 0.1 M CHES/sodium hydroxide, pH 9.5
Methanotorris igneus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Methanocaldococcus jannaschii
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Saccharomyces cerevisiae
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
Methanotorris igneus
-
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography and Superdex 200 gel filtration
Methanocaldococcus jannaschii
Ni-NTA column chromatography and Superdex 200 gel filtration
Saccharomyces cerevisiae
Ni-NTA column chromatography and Superdex 200 gel filtration
Methanotorris igneus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Methanocaldococcus jannaschii
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Saccharomyces cerevisiae
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS]
-
737721
Methanotorris igneus
2-[(2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene]ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O
-
-
-
?
Other publictions for EC 2.8.1.10
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737721
Zhang
Structural Basis for iron-medi ...
Methanocaldococcus jannaschii, Methanotorris igneus, Saccharomyces cerevisiae
Biochemistry
55
1826-1838
2016
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739358
Li
THI1, a thiamine thiazole synt ...
Arabidopsis thaliana
Plant Physiol.
170
1090-1104
2016
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739422
Yu
The thiG gene is required for ...
Xanthomonas oryzae pv. oryzae, Xanthomonas oryzae pv. oryzae ZJ173
PLoS ONE
10
e0134237
2015
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723976
Rohini
Structural insights on Mycobac ...
Mycobacterium tuberculosis
Appl. Biochem. Biotechnol.
169
1790-1798
2013
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715306
Hazra
A missing enzyme in thiamin th ...
Bacillus subtilis
J. Am. Chem. Soc.
133
9311-9319
2011
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716340
Chatterjee
Saccharomyces cerevisiae THI4p ...
Saccharomyces cerevisiae
Nature
478
542-546
2011
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708874
Hazra
Biosynthesis of the thiamin th ...
Bacillus subtilis
J. Am. Chem. Soc.
131
3225-3229
2009
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715251
Chatterjee
Biosynthesis of the thiamin-th ...
Saccharomyces cerevisiae
J. Am. Chem. Soc.
130
11394-11398
2008
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715486
Kriek
Thiazole synthase from Escheri ...
Escherichia coli
J. Biol. Chem.
282
17413-17423
2007
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714159
Jurgenson
Structural insights into the f ...
Saccharomyces cerevisiae
Biochemistry
45
11061-11070
2006
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707468
Settembre
Thiamin biosynthesis in Bacill ...
Bacillus subtilis
Biochemistry
43
11647-11657
2004
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715242
Dorrestein
The biosynthesis of the thiazo ...
Bacillus subtilis
J. Am. Chem. Soc.
126
3091-3096
2004
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715465
Leonardi
Thiamine biosynthesis in Esche ...
Escherichia coli
J. Biol. Chem.
279
17054-17062
2004
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707466
Park
Biosynthesis of the thiazole m ...
Bacillus subtilis
Biochemistry
42
12430-12438
2003
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