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Literature summary for 2.7.9.5 extracted from

  • Orzechowski, S.; Grabowska, A.; Sitnicka, D.; Siminska, J.; Felus, M.; Dudkiewicz, M.; Fudali, S.; Sobczak, M.
    Analysis of the expression, subcellular and tissue localisation of phosphoglucan, water dikinase (PWD/GWD3) in Solanum tuberosum L.: A bioinformatics approach for the comparative analysis of two alpha-glucan, water dikinases (GWDs) from Solanum tuberosum L. (2013), Acta Physiol. Plant., 35, 483-500.
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain JM109 Solanum tuberosum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
125000
-
 x * 125000, SDS-PAGE Solanum tuberosum
132270
-
 x * 132270, calculated from amino acid sequence Solanum tuberosum

Organism

Organism UniProt Comment Textmining
Solanum tuberosum D2JRZ6 cultivar Russet Burbank
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, amylose resin column chromatography, and Sephadex G-25 gel filtration Solanum tuberosum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Solanum tuberosum
-
tuber
-
 Solanum tuberosum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [phospho-alpha-glucan] + H2O
-
 Solanum tuberosum AMP + O-phospho-[phospho-alpha-glucan] + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 125000, SDS-PAGE Solanum tuberosum
? x * 132270, calculated from amino acid sequence Solanum tuberosum

Synonyms

Synonyms Comment Organism
GWD3 isoform Solanum tuberosum
PWD isoform Solanum tuberosum

pI Value

Organism Comment pI Value Maximum pI Value
Solanum tuberosum calculated from amino acid sequence
-
 5.8

Expression

Organism Comment Expression
Solanum tuberosum the lowest level isoform GWD3 expression is 4 h after turning the light off, and it was more than 2.5times lower than the maximal expression level down
Solanum tuberosum the expression of isoform GWD3 during the diurnal cycle increases until 13 h of illumination, when it reaches its maximal level up