Cloned (Comment) | Organism |
---|---|
codon-optimized coding regions of PPDK from Flaveria trinervia stripped of the chloroplast transport sequence are cloned into the multiple cloning site of a pET-16b vector (Novagen) containing a His10 tag and coding for a Tobacco Etch Virus protease cleavage site. Escherichia coli BL21 (DE3) cells are transformed with this plasmid | Flaveria trinervia |
Crystallization (Comment) | Organism |
---|---|
crystals of the FtPPDK-pyruvate-ADP complex are obtained using the sitting drop vapor diffusion method at 21°C | Flaveria trinervia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | Flaveria trinervia | essential enzyme of C4 photosynthetic pathway | AMP + phosphoenolpyruvate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flaveria trinervia | P22221 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Flaveria trinervia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
Flaveria trinervia | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | essential enzyme of C4 photosynthetic pathway | Flaveria trinervia | AMP + phosphoenolpyruvate + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
Flaveria trinervia |
General Information | Comment | Organism |
---|---|---|
physiological function | essential enzyme of C4 photosynthetic pathway | Flaveria trinervia |