Cloned (Comment) | Organism |
---|---|
the His6-tagged catalytic domain is expressed in Escherichia coli BL21(DE3) and C43(DE3) cells | Salmonella enterica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CHAPS | 62.4% relative activity at 0.5 mM | Salmonella enterica | |
KCl | KCl inhibits the enzyme domain activity, particularly at concentrations higher than 200 mM salt that result in more than 50% inhibition | Salmonella enterica | |
Mg2+ | no activity at 150 mM | Salmonella enterica | |
n-dodecyl-beta-D-maltoside | 81.5% relative activity at 0.5 mM | Salmonella enterica | |
NaCl | NaCl inhibits the enzyme domain activity, particularly at concentrations higher than 200 mM salt that result in more than 50% inhibition | Salmonella enterica | |
octylglucoside | 65.5% relative activity at 0.5 mM | Salmonella enterica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00055 | - |
UDP-alpha-D-galactose | in 50 mM Tris-HCl (pH 8.5), 25 mM MgCl2, 1% (w/v) CHAPS, at 37°C | Salmonella enterica | |
0.0785 | - |
undecaprenyl phosphate | in 50 mM Tris-HCl (pH 8.5), 25 mM MgCl2, 1% (w/v) CHAPS, at 37°C | Salmonella enterica |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Salmonella enterica | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | without adding MgCl2, no activity can be detected. Activity is optimal at 25 mM | Salmonella enterica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, His6-tagged catalytic domain, SDS-PAGE | Salmonella enterica |
Organic Solvent | Comment | Organism |
---|---|---|
Triton X-100 | 3.5% relative activity at 1 mM | Salmonella enterica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-bound chelating Sepharose column chromatography, and gel filtration | Salmonella enterica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | minimal activity is detected with the shorter polyisoprenyl phosphates like C10-P, C15-P, C20-P and C35-P at 0.28, 0.91, 1 and 1.8% of C55-undecaprenyl phosphate activity, respectively. C50-phosphate, however, results in 49.53% activity of C55-phosphate | Salmonella enterica | ? | - |
? | |
UDP-alpha-D-galactose + undecaprenyl phosphate | specific activity with C55-undecaprenyl phosphate | Salmonella enterica | UMP + alpha-D-galactosyl-diphosphoundecaprenol | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, His6-tagged catalytic domain, SDS-PAGE | Salmonella enterica |
Synonyms | Comment | Organism |
---|---|---|
UDP-galactose:Und-P galactose-1-phosphate transferase | - |
Salmonella enterica |
WbaP | - |
Salmonella enterica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Salmonella enterica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Salmonella enterica |