Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methanocaldococcus jannaschii |
Protein Variants | Comment | Organism |
---|---|---|
S211A | mutant enzyme has the same specific activity as the wild type enzyme | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
lactyl-2-diphospho-5'-guanosine | pH 7.0, 37°C | Methanocaldococcus jannaschii | |
0.032 | - |
7,8-didemethyl-8-hydroxy-5-deazariboflavin | pH 7.0, 37°C | Methanocaldococcus jannaschii | |
0.515 | - |
lactyl-2-diphospho-5'-adenosine | pH 7.0, 37°C | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | isolated CofD enzyme is not activated by the addition of 0-4 mM MgCl2. The inactivation by EDTA can be completely reversed by the addition of excess Mg2+ but not by the addition of Zn2+ or Mn2+ | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34400 | - |
2 * 34400, SDS-PAGE | Methanocaldococcus jannaschii |
66700 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin | Methanocaldococcus jannaschii | the reaction is the fourth step in the biosynthesis of coenzyme F420 | guanosine 5'-phosphate + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58653 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Methanocaldococcus jannaschii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = GMP + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.36 | - |
- |
Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
lactyl-2-diphospho-5'-adenosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin | catalysis does not appear to proceed via a covalent intermediate | Methanocaldococcus jannaschii | adenosine 5'-phosphate + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | ? | |
lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin | the reaction is the fourth step in the biosynthesis of coenzyme F420 | Methanocaldococcus jannaschii | guanosine 5'-phosphate + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | ? | |
lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin | catalysis does not appear to proceed via a covalent intermediate. Lactyl-2-diphospho-5'-guanosine is the preferred substrate. The substrate binding site of CofD is very specific for 7,8-didemethyl-8-hydroxy-5-deazariboflavin | Methanocaldococcus jannaschii | guanosine 5'-phosphate + coenzyme F420-0 | coenzyme F420-0 is coenzyme F420 without glutamic acid | ? | |
additional information | to a lesser extent CofD also catalyzes a number of additional reactions that include the formation of 7,8-didemethyl-8-hydroxy-5-deazariboflavin 5'-phosphate, when the enzyme is incubated with 7,8-didemethyl-8-hydroxy-5-deazariboflavin and GDP, GTP, diphosphate, or tripolyphosphate, and the hydrolysis of F420-0 (F420 without glutamic acids) to 7,8-didemethyl-8-hydroxy-5-deazariboflavin | Methanocaldococcus jannaschii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 34400, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
CofD | - |
Methanocaldococcus jannaschii |
lactyl-2-diphospho-(5')guanosine:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
lactyl-2-diphospho-(5')guanosine:Fo 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
LPPG:7,8-didemethyl-8-hydroxy-5-deazariboflavin 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
LPPG:Fo 2-phospho-L-lactate transferase | - |
Methanocaldococcus jannaschii |
MJ1256 | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
24 h, completely stable | Methanocaldococcus jannaschii |
110 | - |
30 min, complete inactivation | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Methanocaldococcus jannaschii |