KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
3-hydroxy-4-methylanthranilate | at pH 8.0 and 28°C | Streptomyces anulatus | |
0.005 | - |
3-hydroxy-4-methylbenzylate | at pH 8.0 and 28°C | Streptomyces anulatus | |
0.018 | - |
3-Hydroxy-anthranilate | at pH 8.0 and 28°C | Streptomyces anulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Streptomyces anulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxy-4-methylanthranilate | Streptomyces anulatus | - |
diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? | |
ATP + 3-hydroxy-4-methylanthranilate | Streptomyces anulatus X2-18 | - |
diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces anulatus | - |
- |
- |
Streptomyces anulatus X2-18 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
polymin P precipitation, DEAE-cellulose column chromatography, ammonium sulfate precipitation, Aca-44 gel filtration, aminohexyl Sepharose column chromatography, Aca-54 gel filtration, and Mono Q column chromatography | Streptomyces anulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.024 | - |
crude extract, at pH 8.0 and 28°C | Streptomyces anulatus |
0.38 | - |
after 15.34fold purification, at pH 8.0 and 28°C | Streptomyces anulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-hydroxy-4-methylanthranilate | - |
Streptomyces anulatus | diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? | |
ATP + 3-hydroxy-4-methylanthranilate | - |
Streptomyces anulatus X2-18 | diphosphate + 3-hydroxy-4-methylanthranilyl-adenylate | - |
? | |
ATP + 3-hydroxy-4-methylbenzylate | - |
Streptomyces anulatus | diphosphate + 3-hydroxy-4-methylbenzyl-adenylate | - |
? | |
ATP + 3-hydroxy-4-methylbenzylate | - |
Streptomyces anulatus X2-18 | diphosphate + 3-hydroxy-4-methylbenzyl-adenylate | - |
? | |
ATP + 3-hydroxy-anthranilate | - |
Streptomyces anulatus | diphosphate + 3-hydroxy-anthranilyl-adenylate | - |
? | |
ATP + 3-hydroxy-anthranilate | - |
Streptomyces anulatus X2-18 | diphosphate + 3-hydroxy-anthranilyl-adenylate | - |
? | |
additional information | the enzyme does not act as an acyl-coenzyme A ligase and has no activity with benzoic acid, 2-aminobenzoic acid, 3-aminobenzoic acid, 4-aminobenzoic acid, 2-hydroxybenzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 3-methylbenzoic acid, and 4-methylbenzoic acid | Streptomyces anulatus | ? | - |
? | |
additional information | the enzyme does not act as an acyl-coenzyme A ligase and has no activity with benzoic acid, 2-aminobenzoic acid, 3-aminobenzoic acid, 4-aminobenzoic acid, 2-hydroxybenzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, 2,3-dihydroxybenzoic acid, 3-methylbenzoic acid, and 4-methylbenzoic acid | Streptomyces anulatus X2-18 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Streptomyces anulatus |
Synonyms | Comment | Organism |
---|---|---|
ACMS I | - |
Streptomyces anulatus |
actinomycin synthetase I | - |
Streptomyces anulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
3-hydroxy-4-methylanthranilate | at pH 8.0 and 28°C | Streptomyces anulatus | |
0.005 | - |
3-Hydroxy-anthranilate | at pH 8.0 and 28°C | Streptomyces anulatus | |
0.007 | - |
3-hydroxy-4-methylbenzylate | at pH 8.0 and 28°C | Streptomyces anulatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
3-Hydroxy-anthranilate | at pH 8.0 and 28°C | Streptomyces anulatus | |
1.1 | - |
3-hydroxy-4-methylbenzylate | at pH 8.0 and 28°C | Streptomyces anulatus | |
7.8 | - |
3-hydroxy-4-methylanthranilate | at pH 8.0 and 28°C | Streptomyces anulatus |