Literature summary for 2.7.7.87 extracted from

Deutsch, C.; El Yacoubi, B.; de Crecy-Lagard, V.; Iwata-Reuyl, D.
Biosynthesis of threonylcarbamoyl adenosine (t6A), a universal tRNA nucleoside (2012), J. Biol. Chem., 287, 13666-13673.

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Cloned(Commentary)

Cloned (Comment)	Organism
yrdC, yjeE and yeaZ genes from Escherichia coli expressed as N-terminal His6-fusion proteins, YgjD N-terminal as His6-SUMO fusion protein in Escherichia coli	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-threonine + ATP + bicarbonate	beside YrdC, proteins YgjD, YjeE and YeaZ are necessary and sufficient for t6A biosynthesis in vitro	Escherichia coli	L-threonylcarbamoyladenylate + diphosphate + H2O	L-threonylcarbamoyladenylate i.e. t6A	?
additional information	t6A is a universally conserved tRNA modification found at position 37 in the anticodon loop of a subset of tRNA, structural studies predict an important role for t6A in translational fidelity	Escherichia coli	?	-	?

Synonyms

Synonyms	Comment	Organism
YrdC	-	Escherichia coli

General Information

General Information	Comment	Organism
physiological function	t6A is a universally conserved tRNA modification found at position 37 in the anticodon loop of a subset of tRNA, structural studies predict an important role for t6A in translational fidelity	Escherichia coli

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Release 2023.1 (January 2023)