Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21(DE3) cells transformed with a pET21 derivative (Novagen) vector containing the Escherichia coli yrdC gene | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + ATP + bicarbonate | Saccharomyces cerevisiae | results indicate that the universally conserved SUA5 family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine | L-threonylcarbamoyladenylate + diphosphate + H2O | threonylcarbamoyladenosine i.e. t6A | ? | |
L-threonine + ATP + bicarbonate | Escherichia coli | results indicate that the universally conserved YrdC family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine | L-threonylcarbamoyladenylate + diphosphate + H2O | threonylcarbamoyladenosine i.e. t6A | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P45748 | - |
- |
Saccharomyces cerevisiae | P32579 | - |
- |
Purification (Comment) | Organism |
---|---|
yrdC protein purified by Ni-NTA affinity chromatography, dialyzed against phosphate-buffered saline and concentrated using a YM-3 Centriprep filter | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-threonine + ATP + bicarbonate | results indicate that the universally conserved SUA5 family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine | Saccharomyces cerevisiae | L-threonylcarbamoyladenylate + diphosphate + H2O | threonylcarbamoyladenosine i.e. t6A | ? | |
L-threonine + ATP + bicarbonate | results indicate that the universally conserved YrdC family is involved in the biosynthesis of N6-threonylcarbamoyl adenosine | Escherichia coli | L-threonylcarbamoyladenylate + diphosphate + H2O | threonylcarbamoyladenosine i.e. t6A | ? |
Synonyms | Comment | Organism |
---|---|---|
SUA5 | - |
Saccharomyces cerevisiae |
YrdC | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
physiological function | threonylcarbamoyladenosine is a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro | Escherichia coli |
physiological function | threonylcarbamoyladenosine is a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro | Saccharomyces cerevisiae |