Literature summary for 2.7.7.84 extracted from

Koul, A.; Deo, S.; Booy, E.P.; Orriss, G.L.; Genung, M.; McKenna, S.A.
Impact of double-stranded RNA characteristics on the activation of human 2'-5'-oligoadenylate synthetase 2 (OAS2) (2020), Biochem. Cell Biol., 98, 70-82 .

Search for more literature for 2.7.7.84

Activating Compound

Activating Compound	Comment	Organism	Structure
dsRNA	OAS1 can be catalytically activated by dsRNA of any length greater than 19 bp. Highly structured viral RNAs are established OAS1 activators, but they are not able to activate OAS2 enzymatic activity based on the lack of extended stretches of dsRNA of greater than 35 bp. In-vitro transcribtion of WNV 5' TR, WNV 3' TR, VAI, VAIDELTATS, HIV-TAR, and EBER-1 from linearized plasmids using T7 polymerase, with the exceptions of HIV-TAR and EBER-1, all viral RNAs can activate OAS1 to some extent. Whereas 5'-TR and 3'-TR achieve potent activation, VAI and VAIDELTATS are only modestly above baseline	Homo sapiens
dsRNA	OAS2 shows a marked increase in activity with increasing dsRNA length with a minimum requirement of 35 bp. Activation of OAS2 is also more efficient when the dsRNA contained 3'-overhangs, despite no significant impact on binding affinity. The OAS2 activation potential is dependent on dsRNA length, kinetic analysis, overview. In-vitro transcribtion of WNV 5' TR, WNV 3' TR, VAI, VAIDELTATS, HIV-TAR, and EBER-1 from linearized plasmids using T7 polymerase, none of the RNAs can activate isozyme OAS2	Homo sapiens
additional information	highly structured viral RNAs that are established OAS1 activators are not able to activate OAS2 enzymatic activity based on the lack of extended stretches of dsRNA of greater than 35 bp. Phosphorylation state of the 5' end of dsRNA activators does not affect OAS2 activation	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
gene OAS1, recombinant expression of His-tagged OAS1 p42 isoform in Escherichia coli strain BL21(DE3)RIL	Homo sapiens
gene OAS2, recombinant expression of N-terminally FLAG-tagged OAS1 p69 isoform in Escherichia coli, and of N-terminally FLAG-tagged OAS2 domain I (DI1-331) or domain II (DII332-687), recombinant expression of N-terminally FLAG-tagged OAS2 in HEK-293T cells	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3 ATP	Homo sapiens	-	pppA2'p5'A2'p5'A + 2 diphosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P00973	-	-
Homo sapiens	P29728	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant FLAG-tagged full-length enzyme and enzyme domains I and II from Escherichia coli by immunoaffinity chromatography using a anti-DYKDDDDK G1 affinity resin, followed by dialysis and gel filtration	Homo sapiens
recombinant His-tagged OAS1 p42 isoform from Escherichia coli strain BL21(DE3)RIL by nickel affinity chromatography, tag cleavage by TEV protease, and dialysis	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
HEK-293T cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3 ATP	-	Homo sapiens	pppA2'p5'A2'p5'A + 2 diphosphate	-	?
additional information	catalytic activity of OAS proteins incubated with polyriboinosinic polyribocytidylic acid [poly (I:C)] in a buffered solution with ATP and MgCl2 at 37°C	Homo sapiens	?	-	-

Subunits

Subunits	Comment	Organism
?	x * 39200, recombinant FLAG-tagged OAS2 domain I, SDS-PAGE, x * 41500, FLAG-tagged OAS2 domain II, x * 69000, recombinant full-length FLAG-tagged OAS2, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
2'-5'-oligoadenylate synthetase 1	-	Homo sapiens
2'-5'-oligoadenylate synthetase 2	-	Homo sapiens
OAS1	-	Homo sapiens
OAS2	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

Expression

Organism	Comment	Expression
Homo sapiens	isozyme OAS2 is interferon-inducible	up

General Information

General Information	Comment	Organism
evolution	isozyme OAS1 belongs to the 2'-5'-oligoadenylate synthetases (OAS) family of interferon-inducible enzymes that act as pattern recognition receptors (PRR) to bind double-stranded RNA (dsRNA) during viral infection. The C-terminal catalytic domain of OAS2 (DII) shares strong sequence homology and catalytic aspartic acids (D408, D410, D481) with OAS1	Homo sapiens
evolution	isozyme OAS2 belongs to the 2'-5'-oligoadenylate synthetases (OAS) family of interferon-inducible enzymes that act as pattern recognition receptors (PRR) to bind double-stranded RNA (dsRNA) during viral infection. The C-terminal catalytic domain of OAS2 (DII) shares strong sequence homology and catalytic aspartic acids (D408, D410, D481) with OAS1iral infection	Homo sapiens
physiological function	different dsRNA length requirement of OAS2 suggests non-overlapping biological functions	Homo sapiens

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Release 2023.1 (January 2023)