Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus shibatae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
ATP | pH 9.0, 70°C, the initial velocity is a linear function of ATP from 0.015 to 0.2 mM, suugesting that the Km for ATP in presence of each UTP, GTP and CTP is much higher than 0.03 mM | Saccharolobus shibatae | |
0.03 | - |
CTP | pH 9.0, 70°C, in presence of 0.1 mM each of UTP, ATP and GTP | Saccharolobus shibatae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
x * 48000, SDS-PAGE | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | P77978 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a tRNA precursor + 2 CTP + ATP | - |
Saccharolobus shibatae | a tRNA with a 3'CCA end + 3 diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, SDS-PAGE | Saccharolobus shibatae |
Synonyms | Comment | Organism |
---|---|---|
ATP(CTP):tRNA nucleotidyl transferase | - |
Saccharolobus shibatae |
CCA-adding enzyme | - |
Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Saccharolobus shibatae |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
weak activity at 50°C, no activity at 37°C | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Saccharolobus shibatae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | the enzyme exhibits substantial activity over a broad range from pH 7 to 10 | Saccharolobus shibatae |