Literature summary for 2.7.7.7 extracted from

Zhang, L.; Jiang, D.; Shi, H.; Wu, M.; Gan, Q.; Yang, Z.; Oger, P.
Characterization and application of a family B DNA polymerase from the hyperthermophilic and radioresistant euryarchaeon Thermococcus gammatolerans (2020), Int. J. Biol. Macromol., 156, 217-224 .

Search for more literature for 2.7.7.7

Application

Application	Comment	Organism
molecular biology	the enzyme can be used in routine PCR	Thermococcus gammatolerans

Cloned(Commentary)

Cloned (Comment)	Organism
-	Thermococcus gammatolerans

Inhibitors

Inhibitors	Comment	Organism	Structure
EDTA	Mg2+ and Mn2+, can restore activity	Thermococcus gammatolerans
NaCl	in absence of NaCl, Tga PolB displays 67% polymerization activity. From 50 to 200 mM NaCl, the activity is higher than 90%, but it is significantly reduced at NaCl concentrations above 400 mM	Thermococcus gammatolerans

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	activity is dependent on a divalent cation, among which magnesium ion is optimal	Thermococcus gammatolerans
NaCl	in absence of NaCl, Tga PolB displays 67% polymerization activity. From 50 to 200 mM NaCl, the activity is higher than 90%, but it is significantly reduced at NaCl concentrations above 400 mM	Thermococcus gammatolerans

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn	Thermococcus gammatolerans	-	diphosphate + DNAn+1	-	?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn	Thermococcus gammatolerans DSM 15229	-	diphosphate + DNAn+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermococcus gammatolerans	C5A317	-	-
Thermococcus gammatolerans DSM 15229	C5A317	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermococcus gammatolerans

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn	-	Thermococcus gammatolerans	diphosphate + DNAn+1	-	?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn	the enzyme is able to efficiently bypass uracil in DNA. The enzyme is halted by an AP site in DNA. Tga PolB extends the mismatched ends with reduced efficiencies. It possesses 3'-5' exonuclease activity. The enzyme can efficiently bind to ssDNA and primed DNA, and has a marked preference for primed DNA	Thermococcus gammatolerans	diphosphate + DNAn+1	-	?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn	-	Thermococcus gammatolerans DSM 15229	diphosphate + DNAn+1	-	?
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn	the enzyme is able to efficiently bypass uracil in DNA. The enzyme is halted by an AP site in DNA. Tga PolB extends the mismatched ends with reduced efficiencies. It possesses 3'-5' exonuclease activity. The enzyme can efficiently bind to ssDNA and primed DNA, and has a marked preference for primed DNA	Thermococcus gammatolerans DSM 15229	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
Tga PolB	-	Thermococcus gammatolerans
TGAM_RS07365	-	Thermococcus gammatolerans

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
95	-	1 h, retains 93% of its activity. 80% loss of activity after 2 h	Thermococcus gammatolerans

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	9	-	Thermococcus gammatolerans

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Release 2023.1 (January 2023)