Application | Comment | Organism |
---|---|---|
analysis | the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications | Acidianus infernus |
analysis | the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications | Sulfurisphaera ohwakuensis |
analysis | the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications | Sulfolobus tengchongensis |
analysis | the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications | Saccharolobus shibatae |
analysis | the enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the polymerase chain reaction amplification of damaged DNA samples. By using a blend of Taq and Dpo4-like enzymes a polymerase chain amplicon is obtained from ultraviolet-irradiated DNA that is largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in polymerase chain reactions, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications | Stygiolobus azoricus |
Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) | Acidianus infernus |
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) | Sulfurisphaera ohwakuensis |
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) | Sulfolobus tengchongensis |
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) | Saccharolobus shibatae |
overexpressed in Escherichia coli (RW382) lacking umuDC (polV) | Stygiolobus azoricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Acidianus infernus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Sulfurisphaera ohwakuensis | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Sulfolobus tengchongensis | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Saccharolobus shibatae | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Stygiolobus azoricus | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidianus infernus | Q2FA65 | - |
- |
Saccharolobus shibatae | Q2FA62 | - |
- |
Stygiolobus azoricus | Q2FA64 | - |
- |
Sulfolobus tengchongensis | Q2FA66 | - |
- |
Sulfurisphaera ohwakuensis | Q2FA63 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Acidianus infernus |
- |
Sulfurisphaera ohwakuensis |
- |
Sulfolobus tengchongensis |
- |
Saccharolobus shibatae |
- |
Stygiolobus azoricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Acidianus infernus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Sulfurisphaera ohwakuensis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Sulfolobus tengchongensis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Saccharolobus shibatae | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Stygiolobus azoricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq | Acidianus infernus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq | Sulfurisphaera ohwakuensis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq | Sulfolobus tengchongensis | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq | Saccharolobus shibatae | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the enzyme can traverse a wide variety of DNA lesions. The enzyme is moderately processive. It can substitute for Taq in polymerase chain reaction (PCR) and can bypass DNA lesions that normally block Taq | Stygiolobus azoricus | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dpo4-like enzyme | - |
Acidianus infernus |
Dpo4-like enzyme | - |
Sulfurisphaera ohwakuensis |
Dpo4-like enzyme | - |
Sulfolobus tengchongensis |
Dpo4-like enzyme | - |
Saccharolobus shibatae |
Dpo4-like enzyme | - |
Stygiolobus azoricus |