Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | optimal concentration: 12 mM | Thermus caldophilus | |
Mn2+ | can partially replace Mg2+ in activation | Thermus caldophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
gel filtration, SDS-PAGE | Thermus caldophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Thermus caldophilus | - |
diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | Thermus caldophilus GH24 | - |
diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus caldophilus | - |
- |
- |
Thermus caldophilus GH24 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus caldophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Thermus caldophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermus caldophilus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 5'--3' activity | Thermus caldophilus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | no exonuclease 3'--5' activity | Thermus caldophilus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Thermus caldophilus GH24 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | exonuclease 5'--3' activity | Thermus caldophilus GH24 | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | no exonuclease 3'--5' activity | Thermus caldophilus GH24 | diphosphate + DNAn+1 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 95000, SDS-PAGE | Thermus caldophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermus caldophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
70 min, 50% inactivation | Thermus caldophilus |
100 | - |
5 min, 50% inactivation | Thermus caldophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Thermus caldophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.5 | 8.7 | narrow pH range | Thermus caldophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Thermus caldophilus | - |
- |
6.5 |