Cloned (Comment) | Organism |
---|---|
into the vector pT7-7 for expression in Escherichia coli BL21-CodonPlus DE3 cells | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0024 | - |
GTP | apparent KM | Methanocaldococcus jannaschii | |
0.0184 | - |
adenosylcobinamide phosphate | apparent KM | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21600 | - |
monomer, determined by gel filtation and SDS-PAGE | Methanocaldococcus jannaschii |
43000 | - |
dimer, determined by gel filtation | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + adenosylcobinamide phosphate | Methanocaldococcus jannaschii | - |
diphosphate + adenosylcobinamide-GDP | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58517 | MJ1117 open reading frame | - |
Purification (Comment) | Organism |
---|---|
in two steps by hydrophobic interaction and ion-exchange chromatography | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + adenosylcobinamide phosphate | - |
Methanocaldococcus jannaschii | diphosphate + adenosylcobinamide-GDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 21600 | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
CobY | - |
Methanocaldococcus jannaschii |
GTP:adenosylcobinamide-phosphate guanylyltransferase | - |
Methanocaldococcus jannaschii |
MjCobY | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
kinetic assay | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
MjCobY denaturates at 80°C with a concomitant loss of activity | Methanocaldococcus jannaschii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.8 | - |
GTP | - |
Methanocaldococcus jannaschii | |
4.7 | - |
adenosylcobinamide phosphate | - |
Methanocaldococcus jannaschii |