Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
vapor diffusion method at 4°C in hanging drops, crystals belong to space group C2 | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
K213S | effective turnover rate is ca. 0.4% of the rate of wild type, retains capacity to form product with significantly reduced efficiency | Escherichia coli |
K27A | amino acid plays an essential role in catalysis because the effective turnover rate is ca. 0.025% of the rate of wild type enzyme | Escherichia coli |
K27S | amino acid plays an essential role in catalysis because the effective turnover rate is ca. 0.025% of the rate of wild type enzyme | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | essential for activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 2-C-methyl-D-erythritol-4-phosphate | Mycobacterium tuberculosis | - |
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
CTP + 2-C-methyl-D-erythritol-4-phosphate | Plasmodium falciparum | - |
diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
CTP + 2-C-methyl-D-erythritol-4-phosphate | Escherichia coli | third step of biosynthesis | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
CTP + 2-C-methyl-D-erythritol-4-phosphate | Escherichia coli | part of mevalonate-indpendent pathway for isoprenoid biosynthesis | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Mycobacterium tuberculosis | - |
- |
- |
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-nitrilotriacetic acid column | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | cytidyltransferase family. Active substrate site consists of a glycine-rich loop spanning Pro13-Arg20. Three-dimensional structure of enzyme is figured out | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CTP + 2-C-methyl-D-erythritol 4-phosphate | - |
Escherichia coli | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
CTP + 2-C-methyl-D-erythritol-4-phosphate | - |
Mycobacterium tuberculosis | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
CTP + 2-C-methyl-D-erythritol-4-phosphate | - |
Plasmodium falciparum | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
CTP + 2-C-methyl-D-erythritol-4-phosphate | third step of biosynthesis | Escherichia coli | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? | |
CTP + 2-C-methyl-D-erythritol-4-phosphate | part of mevalonate-indpendent pathway for isoprenoid biosynthesis | Escherichia coli | diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | homodimer | Escherichia coli |
dimer | each subunit contains a globular core domain with an alpha/beta sturcture and and one smaller subdomain | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Escherichia coli |