Cloned (Comment) | Organism |
---|---|
genes mth682 and mth683 encode the mthRrp41-mthRrp42 protein complex, cloning and recombinant expression of the His-tagged protein complex in Escherichia coli strain E2566 | Methanothermobacter thermautotrophicus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant mthRrp41-mthRrp42 protein complex, hanging-drop vapour diffusion, 0.001 ml of protein in 25 mM Tris pH 7.5, 0.15 M NaCl, mixed with 0.001 ml of reservoir solution containing 5% 2-propanol, 20% PEG 3350, and 0.1 M succinic acid pH 7.0, X-ray diffraction structure determination and analysis at 2.65 A resolution | Methanothermobacter thermautotrophicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Methanothermobacter thermautotrophicus | 5829 | - |
exosome | the core of the exosome is a versatile multisubunit RNA processing enzyme found in archaea and eukaryotes, which includes a ring of six RNase PH subunits | Methanothermobacter thermautotrophicus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
genes mth682 and mth683 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged mthRrp41-mthRrp42 protein complex from Escherichi coli strain E2566 by nickel affinity chromatography and ultrafiltration | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the reaction proceeds via strongly bound phosphate ions in the phosphorolytic active sites of mthRrp41 protein | Methanothermobacter thermautotrophicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | multisubunit RNA processing enzyme including a ring of six RNase PH subunits | Methanothermobacter thermautotrophicus |
Synonyms | Comment | Organism |
---|---|---|
RNase PH | - |
Methanothermobacter thermautotrophicus |
RNase PH ring | - |
Methanothermobacter thermautotrophicus |
General Information | Comment | Organism |
---|---|---|
additional information | the core of the exosome is a versatile multisubunit RNA processing enzyme found in archaea and eukaryotes, which includes a ring of six RNase PH subunits, all six RNase PH monomers are catalytically active in the homohexameric RNase PH. Modeling of the Mth exosome RNase PH ring, overview | Methanothermobacter thermautotrophicus |