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Literature summary for 2.7.7.52 extracted from

  • Yamashita, S.; Takagi, Y.; Nagaike, T.; Tomita, K.
    Crystal structures of U6 snRNA-specific terminal uridylyltransferase (2017), Nat. Commun., 8, 15788 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.52

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and enzyme in complex with UTP or ATP, hanging drop vapor diffusion method, using 13-15% (w/v) PEG3350 as the precipitant Homo sapiens

Protein Variants

Protein Variants Comment Organism
R779A/R783A the mutations reduce the RNA-binding and uridylylation activities of the enzyme Homo sapiens
R871A/K874A the mutations reduce the RNA-binding activity of the enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000055
-
 U6 snRNAn wild type enzyme, at pH 8.5 and 37°C Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UTP + U6 snRNAn Homo sapiens the enzyme builds or repairs the 3'-oligo-uridylylated tail of U6 snRNA diphosphate + U6 snRNAn+1
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens F5H0R1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, HiTrap heparin column chromatography and Superdex 200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme has no activity with ATP Homo sapiens ?
-
-
UTP + U6 snRNAn the enzyme builds or repairs the 3'-oligo-uridylylated tail of U6 snRNA Homo sapiens diphosphate + U6 snRNAn+1
-
 ?

Synonyms

Synonyms Comment Organism
TUT1
-
 Homo sapiens
TUTase6
-
 Homo sapiens
U6 snRNA-specific terminal uridylyltransferase
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.061
-
 U6 snRNAn wild type enzyme, at pH 8.5 and 37°C Homo sapiens

General Information

General Information Comment Organism
metabolism the U6 snRNA-specific terminal uridylyltransferase is required for pre-mRNA splicing Homo sapiens