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Literature summary for 2.7.7.50 extracted from

  • Hoefer, K.; Li, S.; Abele, F.; Frindert, J.; Schlotthauer, J.; Grawenhoff, J.; Du, J.; Patel, D.; Jaeschke, A.
    Structure and function of the bacterial decapping enzyme NudC (2016), Nat. Chem. Biol., 12, 730-734 .
No PubMed abstract available
Search for more literature for 2.7.7.50

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli One Shot BL21 Star (DE3) cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme in complex with either NAD or NMN, hanging drop vapor diffusion method, using either 0.2 M sodium nitrate, 20% PEG-3350 or 1 M LiCl2, 0.1 M HEPES, pH 7.0, and 10% PEG-6000 Escherichia coli

Protein Variants

Protein Variants Comment Organism
E174Q inactive Escherichia coli
E178Q inactive Escherichia coli
E219Q inactive Escherichia coli
F160A inactive Escherichia coli
P236A the mutant shows 10% of wild type activity Escherichia coli
R69A the mutant shows high activity as compared to the wild type enzyme Escherichia coli
Y124A inactive Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Escherichia coli
Zn2+ contains zinc Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD-capped RNA + H2O Escherichia coli NudC is a single-strand-specific RNA-decapping enzyme with a strong preference for a purine as first nucleotide ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex G200 gel filtration Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5'-NAD-capped RNA + ? NudC is a single-strand-specific RNA-decapping enzyme with a strong preference for a purine as first nucleotide. NudC prefers NAD-RNA over NAD(H) by several orders of magnitude. The enzyme has strong preference for RNAs with at least three unpaired nucleotides at their 5' ends Escherichia coli ?
-
 ?
additional information an RNAI mutant with a paired 5' end, as well as native Escherichia coli 5S rRNA (also possessing a paired 5' end) are resistant to enzyme processing Escherichia coli ?
-
-
NAD(H) + H2O c.f. 3.6.1.22. The enzyme is not very efficient in hydrolyzing NAD Escherichia coli AMP + NMN(H)
-
 ?
NAD-capped RNA + H2O NudC is a single-strand-specific RNA-decapping enzyme with a strong preference for a purine as first nucleotide Escherichia coli ?
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Escherichia coli

Synonyms

Synonyms Comment Organism
NAD-decapping enzyme
-
 Escherichia coli
NudC
-
 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Escherichia coli