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Literature summary for 2.7.7.23 extracted from

  • Wang-Gillam, A.; Pastuszak, I.; Elbein, A.D.
    A 17-amino acid insert changes UDP-N-acetylhexosamine pyrophosphorylase specificity from UDP-GalNAc to UDP-GlcNAc (1998), J. Biol. Chem., 273, 27055-27057.
    View publication on PubMed
Search for more literature for 2.7.7.23

Cloned(Commentary)

Cloned (Comment) Organism
AGX1 and AGX 2 expressed in Escherichia coli as GST fusion protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.38
-
 N-acetyl-alpha-D-galactosamine 1-phosphate AGX1, 37°C, pH 7.5 Homo sapiens
1.3
-
 N-acetyl-alpha-D-galactosamine 1-phosphate AGX2, 37°C, pH 7.5 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-
cytoplasm
-
 Sus scrofa 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE, two bands Sus scrofa
57000
-
 SDS-PAGE, AGX1 expressed in Escherichia coli Homo sapiens
64000
-
 SDS-PAGE, two bands Sus scrofa
64000
-
 SDS-PAGE, AGX2 expressed in Escherichia coli Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + UDP-N-acetyl-D-glucosamine Homo sapiens amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine Sus scrofa amino sugar metabolism UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
GST fusion protein from E. coli Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Sus scrofa
-
sperm
-
 Homo sapiens
-
testis
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + UDP-N-acetyl-D-galactosamine
-
 Sus scrofa UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-galactosamine 2-3 times more active with N-acetyl-D-galactosamine 1-phosphate than with N-acetyl-D-glucosamine 1-phosphate, AGX2 8 times less active with N-acetyl-D-galactosamine 1-phosphate than with N-acetyl-D-glucosamine 1-phosphate Homo sapiens UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 ?
diphosphate + UDP-N-acetyl-D-glucosamine
-
 Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine
-
 Sus scrofa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Homo sapiens UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
diphosphate + UDP-N-acetyl-D-glucosamine amino sugar metabolism Sus scrofa UTP + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 r
UTP + N-acetyl-alpha-D-galactosamine 1-phosphate
-
 Homo sapiens diphosphate + UDP-N-acetyl-D-galactosamine
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 64000 + 1 * 57000 Sus scrofa

Synonyms

Synonyms Comment Organism
AGX1 from human sperm, 2-3 times more active with N-acetyl-D-galactosamine 1-phosphate than with N-acetyl-D-glucosamine 1-phosphate Homo sapiens
AGX2 identical sequence to AGX1 except a 17 amino acid insert at C-terminus, 8 times less active with N-acetyl-D-galactosamine 1-phosphate than with N-acetyl-D-glucosamine 1-phosphate Homo sapiens